A0AA91TXD1 · A0AA91TXD1_WOLPI

Function

function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per monomer.

Features

Showing features for binding site.

137250100150200250300350
TypeIDPosition(s)Description
Binding site147Zn2+ 1 (UniProtKB | ChEBI)
Binding site150Zn2+ 1 (UniProtKB | ChEBI)
Binding site164Zn2+ 2 (UniProtKB | ChEBI)
Binding site167Zn2+ 2 (UniProtKB | ChEBI)
Binding site186Zn2+ 2 (UniProtKB | ChEBI)
Binding site189Zn2+ 2 (UniProtKB | ChEBI)
Binding site200Zn2+ 1 (UniProtKB | ChEBI)
Binding site203Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionheat shock protein binding
Molecular Functionunfolded protein binding
Molecular Functionzinc ion binding
Biological Processchaperone cofactor-dependent protein refolding
Biological ProcessDNA replication
Biological Processprotein refolding
Biological Processresponse to heat

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Chaperone protein DnaJ

Gene names

    • Name
      dnaJ
    • ORF names
      BTO27_03155

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • wAus
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rickettsiales > Anaplasmataceae > Wolbachieae > Wolbachia

Accessions

  • Primary accession
    A0AA91TXD1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Structure

3D structure databases

Family & Domains

Features

Showing features for domain, zinc finger, repeat.

TypeIDPosition(s)Description
Domain5-70J
Domain134-212CR-type
Zinc finger134-212CR-type
Repeat147-154CXXCXGXG motif
Repeat164-171CXXCXGXG motif
Repeat186-193CXXCXGXG motif
Repeat200-207CXXCXGXG motif

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Sequence similarities

Belongs to the DnaJ family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    40,875
  • Last updated
    2024-03-27 v1
  • Checksum
    342D48DBDC84F690
MNKKDYYDLLEVSRNASTDEIKKAYKKLALKYHPDRNPGNKEAEEKFKEVTAAYEVLSDSEKRAGYDRYGHDGASGGFDFSQAGGDFSDIFNDFFGGGFGGSTSRSRTKRSTTGVSGADLRYDLKITLEDAFKGIQAPIHYVTNIKCNTCQGTGSEGAIKPVQCNTCQGSGRIRTQQGFFTIERTCTTCYGEGEIIQNKCKKCGGSGRKRDEVNISVSIPKGIEEGAKVRVSGKGEAGARGGKSGDLYVCVKIATHQIFTRNRADLHCKVPIRMTLAVLGGEIDIQSIDGAKIKVKVPEGTQTGTKLRCREKGMPYMNSHARGDLYVQVIVETLNPKNLTQKQIELLKALEEEEHESVEQKSEGFFGKVKKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MRWX01000023
EMBL· GenBank· DDBJ
PBQ27666.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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