A0AA50DXC1 · A0AA50DXC1_9SYNC

  • Protein
    Flavin-dependent thymidylate synthase
  • Gene
    thyX
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site56FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site76-79dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site79-81FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site87FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site87-91dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site155dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site177FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Active site182Involved in ionization of N3 of dUMP, leading to its activation
Binding site182dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionNADPH binding
Molecular Functionthymidylate synthase (FAD) activity
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-dependent thymidylate synthase
  • EC number
  • Short names
    FDTS
  • Alternative names
    • FAD-dependent thymidylate synthase
    • Thymidylate synthase ThyX
      (TS
      ; TSase
      )

Gene names

    • Name
      thyX
    • ORF names
      NON20_16605

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • B12
  • Taxonomic lineage
    Bacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Merismopediaceae > Synechocystis

Accessions

  • Primary accession
    A0AA50DXC1

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    215
  • Mass (Da)
    25,112
  • Last updated
    2024-01-24 v1
  • Checksum
    8331A97F9D1D3C6E
MDVRFISLTKPEIVIDGEPLSPEGLIAYCARVSSPNQENPNYTKLLQFCIREGHWSIFEMVDMTLEITTTRAIAPQILRHRSFSFQEFSLRYSCATEYECYEARRQDVKNRQNSLDDFDESTKKWFNQAQADVWEKSHQLYEEALAKGIAKECARSILPLNTVTRLYMKGSVRSWIHYFSVRCDQATQKEHREIALAARKIFMEHFPTVAAALEW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP101709
EMBL· GenBank· DDBJ
WLT37454.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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