A0AA50DXC1 · A0AA50DXC1_9SYNC
- ProteinFlavin-dependent thymidylate synthase
- GenethyX
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids215 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.
Catalytic activity
- dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H+ = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP+
Cofactor
Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 56 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | |||
Binding site | 76-79 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 79-81 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | |||
Binding site | 87 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | |||
Binding site | 87-91 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 155 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 177 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | |||
Active site | 182 | Involved in ionization of N3 of dUMP, leading to its activation | |||
Binding site | 182 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | NADPH binding | |
Molecular Function | thymidylate synthase (FAD) activity | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavin-dependent thymidylate synthase
- EC number
- Short namesFDTS
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Merismopediaceae > Synechocystis
Accessions
- Primary accessionA0AA50DXC1
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length215
- Mass (Da)25,112
- Last updated2024-01-24 v1
- Checksum8331A97F9D1D3C6E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP101709 EMBL· GenBank· DDBJ | WLT37454.1 EMBL· GenBank· DDBJ | Genomic DNA |