A0AA49J0M1 · A0AA49J0M1_9PROT

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

1852100200300400500600700800
TypeIDPosition(s)Description
Binding site301Mg2+ (UniProtKB | ChEBI); catalytic
Binding site344Mg2+ (UniProtKB | ChEBI); catalytic
Binding site402Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site405Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      OHM77_03400

Organism names

Accessions

  • Primary accession
    A0AA49J0M1

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain37-118S1 motif
Region402-405Required for zinc-mediated homotetramerization and catalytic activity
Region507-559Disordered
Region571-739Disordered
Compositional bias584-675Basic and acidic residues
Compositional bias688-705Polar residues
Region770-797Disordered
Compositional bias775-796Pro residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    852
  • Mass (Da)
    94,485
  • Last updated
    2024-01-24 v1
  • Checksum
    4AF890274DD865EF
MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKAVITRIEPSLEAAFVDYGAERHGFLPFKEVSRACFKQGIDAGKARIQDALSVGQELIVQVEKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELREVMDRLEVPGGASLIARTAGIGRTYEELQWDLNYLLQLWGAIEGAAKSQSGAFLIYQESSLVIRAIRDYFHADIGEILIDTDEIYEQAQQFMAHVMPGNLARVKRYRDDVPLFSRFQIEHQIESAYSRQVNLPSGGAVVIDHTEALVSVDVNSGRATKGSDIEETALRTNLEAADEVARQLRLRDLGGLIVIDFIDMESARNQREVENRLRDALHFDRARVQTGKISRFGLLELSRQRLRPALAETSYITCPRCTGTGHIRSTESAALHILRILEEEAMKENTGAIHCQVPVDVATFLLNEKRVDIAKIEVRHRINLVIVPNRHLETPQHEIVRLRHDQLNAEDLAIPSYQMAEKAAEEAYQPPSAQIESKAARQEAAVKGITPEQPAPIVEPRPQAAQATRNEAKEGPSIIARIFSFFRGNKAAQEPQPEPKSGRTGRRERGERGARGEDRNDNRRGRNRKEGRENRDEPRREPGERTPRGERSGQRGGERGRQREEQQAEPVQKEGRRQRSREERPAEPRPPRTPRLESMTEAVAEEPASSTSVTEGNAPQTEAQGEGRRRGRRGRGGRGGERRSEESVSAPVETTEAVVVEPVMPKPMPWPVPMPVAAVEEVAAPAVAPPAEEPVAEPAPAPEAPKPAPAAMPPVDLQQAGLVMIETSAEKSNAVSEIVVSSQPLGRKPRPAVVIASEPLQMVETKQD

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias584-675Basic and acidic residues
Compositional bias688-705Polar residues
Compositional bias775-796Pro residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP107246
EMBL· GenBank· DDBJ
WIM06346.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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