A0AA49ECK7 · A0AA49ECK7_9NIDO

  • Protein
    ORF1ab polyprotein
  • Gene
    ORF1ab
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5725
Active site5727
Active site5826
Active site5902
Active site5907
Binding site5965-5971S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6379
Active site6394
Active site6435

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      ORF1ab

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BtSk-AlphaCoV/GX2019-Q156
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae

Accessions

  • Primary accession
    A0AA49ECK7

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1978-1996Helical
Transmembrane2039-2058Helical
Transmembrane2125-2148Helical
Transmembrane2169-2192Helical
Transmembrane2543-2563Helical
Transmembrane2772-2790Helical
Transmembrane2802-2819Helical
Transmembrane2831-2854Helical
Transmembrane2874-2902Helical
Transmembrane3352-3371Helical
Transmembrane3378-3395Helical
Transmembrane3415-3434Helical
Transmembrane3446-3463Helical
Transmembrane3469-3488Helical
Transmembrane3509-3531Helical

Keywords

PTM/Processing

Keywords

Interaction

Subunit

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain112-368CoV Nsp2 N-terminal
Domain387-785CoV Nsp2 middle
Domain776-897CoV Nsp2 C-terminal
Domain898-993Ubiquitin-like
Domain1069-1314Peptidase C16
Domain1292-1459Macro
Domain1638-1693Ubiquitin-like
Domain1699-1965Peptidase C16
Domain2052-21163Ecto
Region2190-2280Y1
Domain2190-2530CoV Nsp3 Y
Region2194-2207ZF1
Region2240-2250ZF2
Region2281-2530CoV-Y
Region2429-2530Y4
Domain2917-3012Nsp4C
Domain3013-3314Peptidase C30
Domain3591-3673RdRp Nsp7 cofactor
Domain3674-3868RdRp Nsp8 cofactor
Domain3869-3976Nsp9 ssRNA-binding
Domain3977-4115ExoN/MTase coactivator
Domain4117-4366NiRAN
Domain4372-4470Nsp12 Interface
Domain4471-5038Nsp12 RNA-dependent RNA polymerase
Domain4718-4880RdRp catalytic
Domain5039-5122CV ZBD
Domain5296-5647+RNA virus helicase C-terminal
Domain5707-5921ExoN
Domain5930-6151N7-MTase
Region6042-6056GpppA-binding
Domain6154-6214Nsp15 N-terminal oligomerization
Domain6215-6332AV-Nsp11N/CoV-Nsp15M
Domain6349-6489NendoU
Domain6493-6789Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,793
  • Mass (Da)
    757,161
  • Last updated
    2024-01-24 v1
  • Checksum
    E1B9851FC4628E3D

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OQ175100
EMBL· GenBank· DDBJ
WCC62135.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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