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A0AA49EC60 · A0AA49EC60_9NIDO

  • Protein
    ORF1ab polyprotein
  • Gene
    orf1ab
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5674
Active site5676
Active site5775
Active site5851
Active site5856
Binding site5914-5920S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6328
Active site6343
Active site6384

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      orf1ab

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BtRf-AlphaCoV/YN2020-Q37
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae

Accessions

  • Primary accession
    A0AA49EC60

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1927-1945Helical
Transmembrane1988-2007Helical
Transmembrane2074-2097Helical
Transmembrane2488-2506Helical
Transmembrane2747-2764Helical
Transmembrane2771-2787Helical
Transmembrane2793-2811Helical
Transmembrane2823-2842Helical
Transmembrane3155-3176Helical
Transmembrane3266-3284Helical
Transmembrane3290-3314Helical
Transmembrane3321-3340Helical
Transmembrane3360-3379Helical
Transmembrane3391-3409Helical
Transmembrane3415-3436Helical
Transmembrane3457-3479Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain112-350CoV Nsp2 N-terminal
Domain774-891CoV Nsp2 C-terminal
Domain892-988Ubiquitin-like
Domain1045-1283Peptidase C16
Domain1261-1428Macro
Domain1597-1652Ubiquitin-like
Domain1659-1911Peptidase C16
Domain2001-20653Ecto
Region2139-2229Y1
Domain2139-2479CoV Nsp3 Y
Region2143-2156ZF1
Region2189-2199ZF2
Region2230-2479CoV-Y
Region2378-2479Y4
Domain2862-2957Nsp4C
Domain2958-3259Peptidase C30
Domain3539-3621RdRp Nsp7 cofactor
Domain3622-3816RdRp Nsp8 cofactor
Domain3817-3924Nsp9 ssRNA-binding
Domain3925-4064ExoN/MTase coactivator
Domain4066-4315NiRAN
Domain4321-4419Nsp12 Interface
Domain4420-4987Nsp12 RNA-dependent RNA polymerase
Domain4988-5071CV ZBD
Domain5235-5596+RNA virus helicase C-terminal
Domain5656-5870ExoN
Domain5879-6100N7-MTase
Region5991-6005GpppA-binding
Domain6103-6163Nsp15 N-terminal oligomerization
Domain6164-6281AV-Nsp11N/CoV-Nsp15M
Domain6298-6438NendoU
Domain6442-6738Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,741
  • Mass (Da)
    750,255
  • Last updated
    2024-01-24 v1
  • MD5 Checksum
    A6F34EB804ED9BF47010769BC84B9DC1

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OQ175057
EMBL· GenBank· DDBJ
WCC61865.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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