A0AA43T4U2 · A0AA43T4U2_9BIFI

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site653ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      OB951_08970

Organism names

Accessions

  • Primary accession
    A0AA43T4U2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif66-76'HIGH' region
Motif650-654'KMSKS' region

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,103
  • Mass (Da)
    124,697
  • Last updated
    2024-01-24 v1
  • Checksum
    2909C8A8F12D647F
MSENNVYPKAAAGEQSANVAPNPSFPKLEESVLDYWEKDDTFQKSIERRPSGDHSQNEFVFFDGPPFANGLPHYGHLLTGYAKDVIPRYQTMKGHKVNRVFGWDTHGLPAELEAQKELGIDSVDQVKEMGIDKFNDACRASVLKYTNEWKDYVHRQARWVDFEHGYKTLNIPYMESVMWAFKQLYDKGLAYQGYRVLPYCPKDQTPLSAHELRMDADVYQDRQDTTVSVAVKLRDEEDAYAVFWTTTPWTVPTNFAIVVGADIDYVEVRPTEGKFAGKKFYLGKPLLGSYAKELGENYEIVRELKGAEMEGWRYYPVFPYFAGDENAVEGKVPGPEGYQIFTADYVDTVEGTGLVHQAPYGEDDMNTLNAKGIKSVDVLDAGCKFTALCPDYEGMYVFDANKPILRNLRAGDGPLERIPEDQRAILFQEKSYVHSYPHCWRCATPLIYKPVSSWFVSVTKIKNRLLELNQEINWIPGNVKDGQFGKWLANARDWSISRNRFWGSPIPVWVSDDPRYPRVDVYGSLDELKADFGDYPRDKDGNVNMHRPYIDELTRVNPDDPTGKSHMHRITDVMDCWFESGSMSFAQYHYPFENKETFEQHFPCDYIVEYIGQTRGWFYVLHIMATALFDKPAFKNVICHGIVLGSDGQKMSKHLRNYPDVNGVFNDFGSDAMRWFLMSSPILRGGNLIVTADGIRDTVRQIMLPVWSSYYFFTLYANAANNGAGFDARALHADEVAALPEMDRYLLARTRRLIEKTQHSLDDFLISDACEAVSDFIDMLTNWYIRNNRDRFWNEDANAFNTLYTVLEAFMRVIAPLAPMEAEAVWRGLTGGESVHLADWPFLADEQTGEASELGRVLVDDPALVDAMEKVREVVSGTLSMRKTKQIRVRQPLSKLTVVVENTAAVAAYDEILKSELNVKNVELCTLEDAEAQGLKIINELRVNARVAGKRLRKDVQFAIKASKSGAWHVNAEGAPVCETPNGEIVLEEGEYELINSVEEKNAEEAANSVSAALPTGGFVILDTELNDDLIAEGYARDVIRAVQDARKAADLQISDRIALKLIVPAEDVTKVEQFKELVSSETLATSFEVTAGDELNVEVAKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAOPMH010000012
EMBL· GenBank· DDBJ
MDH7890719.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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