A0AA42FQ04 · A0AA42FQ04_9GAMM
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids305 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- H2O + N-acetyl-D-muramate 6-phosphate = (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
Pathway
Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.
Amino-sugar metabolism; N-acetylmuramate degradation.
Cell wall biogenesis; peptidoglycan recycling.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 88 | Proton donor | ||||
Sequence: E | ||||||
Active site | 119 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbohydrate derivative binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | ether hydrolase activity | |
Biological Process | amino sugar catabolic process | |
Biological Process | peptidoglycan turnover |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Morganellaceae > Providencia
Accessions
- Primary accessionA0AA42FQ04
Proteomes
Expression
Induction
Induced by MurNAc 6-phosphate that releases the repressor MurR from the DNA. Repressed by MurR in the absence of MurNAc 6-phosphate.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 60-223 | SIS | ||||
Sequence: IAECLKQGGRLFYIGAGTSGRLAVLDSAECPPTFGTSPELVQSIIAGGQDAMLKAVENIEDSPEAAIEELKKRQLCGKDVVVGIAASGRTPFTLAGIDYANGLGALTVAITTRGRGLLSHIAKIAIAPDVGPEVLSGSTRMKSGTAQKMILGMLSTCVMGRLGR |
Sequence similarities
Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)32,542
- Last updated2024-01-24 v1
- ChecksumCE96B13399495F14