A0AA37X0M7 · A0AA37X0M7_9RHOB
- ProteinPyruvate carboxylase
- GenepycA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1146 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- ATP + hydrogencarbonate + pyruvate = ADP + H+ + oxaloacetate + phosphate
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 119 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 203 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 296 | |||||
Sequence: R | ||||||
Binding site | 542 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 614 | substrate | ||||
Sequence: R | ||||||
Binding site | 711 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 740 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 742 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 875 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Paracoccaceae > Cypionkella
Accessions
- Primary accessionA0AA37X0M7
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 711 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1112 | N6-biotinyllysine | ||||
Sequence: K |
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-456 | Biotin carboxylation | ||||
Sequence: EFKKILIANRGEIAIRVMRAANEMGKKTVAVYAEEDKLSLHRFKADEAYLIGEGLSPVGAYLSIPEIIRVARACGADAIHPGYGLLSENPDFVDACDQAGITFIGPKADTMRALGDKASARRVAMEAGVPVIPATEVLGDDFDAIKKEAAAVGYPLMLKASWGGGGRGMRPILGPDELVDKVREGRREAEAAFGNGEGYLEKMIIRARHVEVQILGDKHGAIYHLYERDCTVQRRNQKVVERAPAPYLTDAQRAEVCELGRKICAHVGYECAGTVEFLMDMDNQQFYFIEVNPRVQVEHTVTEQVTGIDIVQAQILIAEGKTLAEATGKPAQSDIHLTGHALQCRVTTEDPQNNFIPDYGRLTAYRSATGNGIRLDGGTAYAGGVITRYYDSLLVKVTATAQTPKAAIARMDRALREFRIRGVSTNIEFVINLLKHPTFLDDTYTTKFIDTTPD | ||||||
Domain | 123-321 | ATP-grasp | ||||
Sequence: RRVAMEAGVPVIPATEVLGDDFDAIKKEAAAVGYPLMLKASWGGGGRGMRPILGPDELVDKVREGRREAEAAFGNGEGYLEKMIIRARHVEVQILGDKHGAIYHLYERDCTVQRRNQKVVERAPAPYLTDAQRAEVCELGRKICAHVGYECAGTVEFLMDMDNQQFYFIEVNPRVQVEHTVTEQVTGIDIVQAQILIAE | ||||||
Region | 482-505 | Disordered | ||||
Sequence: HPETAGRPKPHAEARTPRAPKAHA | ||||||
Compositional bias | 487-501 | Basic and acidic residues | ||||
Sequence: GRPKPHAEARTPRAP | ||||||
Domain | 533-801 | Pyruvate carboxyltransferase | ||||
Sequence: LLLTDTTMRDGHQSLLATRMRSIDMIKVAPAYAANLPQLFSVECWGGATFDVAYRFLQECPWQRLRDLRALMPNVMTQMLLRASNGVGYTNYPDNVVQAFVKQAALTGVDVFRVFDSLNWVENMRVAMDAVIEANKVCEGTVCYTGDMLDPDRAKYDLKYYVDTAKQLKAAGAHVLGLKDMAGLLKPAAAKQLIKTLKQEVGLPIHFHTHDTSGAAAATILAAADAGVDCVDAAMDAFSGGTSQPCLGSIVEALRHTDRDTGLDMAAIR | ||||||
Domain | 1071-1146 | Lipoyl-binding | ||||
Sequence: KAVEGNAGHIGAPMPGSIASVGVVVGQKVSAGDLLLTIEAMKMETGIHADRAAVVKALHVQPGSQIDAKDLLVELE |
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,146
- Mass (Da)124,861
- Last updated2024-01-24 v1
- ChecksumA5C196D4F225B7B1
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 487-501 | Basic and acidic residues | ||||
Sequence: GRPKPHAEARTPRAP |