A0AA36GZN9 · A0AA36GZN9_CYLNA
- ProteinSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids961 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- ATP + D-glucose = ADP + D-glucose 6-phosphate + H+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.
Carbohydrate metabolism; hexose metabolism.
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 581 | GTP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Site | 608 | Important for substrate specificity | ||||
Sequence: E | ||||||
Binding site | 619-621 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GRG | ||||||
Binding site | 677 | GTP (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 774 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 788 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 839 | substrate; ligand shared with subunit alpha | ||||
Sequence: N | ||||||
Binding site | 896-898 | substrate; ligand shared with subunit alpha | ||||
Sequence: GIV |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | succinate-CoA ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | D-glucose binding | |
Molecular Function | GTP binding | |
Molecular Function | hexokinase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Molecular Function | succinate-CoA ligase (GDP-forming) activity | |
Biological Process | intracellular glucose homeostasis | |
Biological Process | succinyl-CoA metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Strongyloidea > Strongylidae > Cylicocyclus
Accessions
- Primary accessionA0AA36GZN9
Proteomes
Subcellular Location
Interaction
Subunit
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 9-202 | Hexokinase N-terminal | ||||
Sequence: EVQEILQKFVISEDDLHIISEKIELEMEAGLSSVDGSSIAMLPSYVPALPDGSEEGKYVAIDLSGKNLRIMLLTLNGKGREPTAVNNNYIVPNHVMKGTGDQLFTFIVNCLQRFLQEFGLVEANLPIGFVFSYPCELLSIRSARLLWWTKGFDIKDCLRKDVVQLLEEALEMNMSTKAQIKAVMNDTVGQLAAA | ||||||
Domain | 212-447 | Hexokinase C-terminal | ||||
Sequence: IGVVIGYGCNSSYLEKTSRITKFDAEGVGYKHPNMVVVTEWEEFGSKGELDSILTQFDKEIDAASVHQGKQIIDKLTGALYLGELVRRVLSQLVLDRVLFDGQPCEKLDEADAFPTKYISEILAEEDGSYKACRRICDELDVPMHGSSDYHIIRDVCFAVSDRSAAIVAAAISALLRHLEVSRVKIGVGGALIQFHPIYHKLLHDKLVDLAPLCTEWELVPADEGSARGAALIAAV | ||||||
Domain | 563-778 | ATP-grasp fold succinyl-CoA synthetase-type | ||||
Sequence: NLHEYQSKELLHKHGCTVQNFIVASDVEEAREKLKRFEMLFEGDIEYVVKAQILAGGRGKGHFIGGDDKIRGVFITLDRDECLKSIPRMLGKRLVTKQTTKNGVEVKKVMVAEGVPIKRETYVAVLMDRDSNGPVIVASPAGGVDIETVAHETPHLIFKEPIDIEVGITDEQAMKIAKNLQFSGEMAEKAAKEIKLLYKAFISSDATQVEINPFVE | ||||||
Domain | 837-957 | ATP-citrate synthase/succinyl-CoA ligase C-terminal | ||||
Sequence: LVNGAGLAMATMDIIKLKGGEPANFLDVGGTVTEEQVYQAFRIITEDAKVKCILVNIFGGIVNCATIAKGVIKACEKIKLKVPLVVRLQGTNVDEARRILKESGLPIISAGDLNDAAEKAV |
Sequence similarities
Belongs to the hexokinase family.
Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length961
- Mass (Da)106,064
- Last updated2024-01-24 v1
- Checksum0F982073CB3935D1
Keywords
- Technical term