A0A9X7U7X0 · A0A9X7U7X0_SPHYA
- Protein6,7-dimethyl-8-ribityllumazine synthase
- GeneribH
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids140 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H+
Pathway
Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 42-44 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 66-68 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 71-72 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | |||
Active site | 74 | Proton donor | |||
Binding site | 98 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | |||
Binding site | 112 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | riboflavin synthase complex | |
Molecular Function | 6,7-dimethyl-8-ribityllumazine synthase activity | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6,7-dimethyl-8-ribityllumazine synthase
- EC number
- Short namesDMRL synthase ; LS ; Lumazine synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium
Accessions
- Primary accessionA0A9X7U7X0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length140
- Mass (Da)14,939
- Last updated2023-11-08 v1
- Checksum9E781B024279746B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP060122 EMBL· GenBank· DDBJ | QNG45391.1 EMBL· GenBank· DDBJ | Genomic DNA |