A0A9X3AR32 · A0A9X3AR32_9ENTR
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids369 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s2U34, the first step of tRNA-mnm5s2U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-19 | ATP (UniProtKB | ChEBI) | |||
Binding site | 38 | ATP (UniProtKB | ChEBI) | |||
Active site | 103 | Nucleophile | |||
Binding site | 128 | ATP (UniProtKB | ChEBI) | |||
Site | 129 | Interaction with tRNA | |||
Active site | 200 | Cysteine persulfide intermediate | |||
Site | 345 | Interaction with tRNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | sulfurtransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Dryocola
Accessions
- Primary accessionA0A9X3AR32
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Disulfide bond | 103↔200 | Alternate | |||
Keywords
- PTM
Interaction
Subunit
Interacts with TusE.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 98-100 | Interaction with target base in tRNA | |||
Region | 150-152 | Interaction with tRNA | |||
Domain | 209-276 | tRNA-specific 2-thiouridylase MnmA-like central | |||
Domain | 287-361 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | |||
Region | 312-313 | Interaction with tRNA | |||
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)41,068
- Last updated2023-11-08 v1
- Checksum18F8BCD403427F3E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JALHAP010000082 EMBL· GenBank· DDBJ | MCT4704165.1 EMBL· GenBank· DDBJ | Genomic DNA |