A0A9X3AR32 · A0A9X3AR32_9ENTR

  • Protein
    tRNA-specific 2-thiouridylase MnmA
  • Gene
    mnmA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s2U34, the first step of tRNA-mnm5s2U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

136950100150200250300350
Type
IDPosition(s)Description
Binding site12-19ATP (UniProtKB | ChEBI)
Binding site38ATP (UniProtKB | ChEBI)
Active site103Nucleophile
Binding site128ATP (UniProtKB | ChEBI)
Site129Interaction with tRNA
Active site200Cysteine persulfide intermediate
Site345Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      MUA00_20515

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • H6W4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Dryocola

Accessions

  • Primary accession
    A0A9X3AR32

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond103↔200Alternate

Keywords

Interaction

Subunit

Interacts with TusE.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region98-100Interaction with target base in tRNA
Region150-152Interaction with tRNA
Domain209-276tRNA-specific 2-thiouridylase MnmA-like central
Domain287-361tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region312-313Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    41,068
  • Last updated
    2023-11-08 v1
  • Checksum
    18F8BCD403427F3E
MSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYKVEGLFMKNWEEDDGEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFEHFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADYIATGHYVRRADVNGKSQLLRGLDGNKDQSYFLYTLGHEQIAQSLFPVGELEKPEVRRIAEELDLITAKKKDSTGICFIGERKFREFLGRYLPAQPGKIVTVDGETIGEHQGLMYHTLGQRKGLGIGGTKEGSEDPWYVVDKDVENNILVVAQGHDHPRLMSVGLIAQQLHWVDRETLSKPLACTVKTRYRQTDIPCTITPIDADRIEVRFEEPVAAVTPGQSAVFYSGEVCLGGGIIEQRLPLID

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JALHAP010000082
EMBL· GenBank· DDBJ
MCT4704165.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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