A0A9X3ADM0 · A0A9X3ADM0_9ENTR
- Protein5-keto-4-deoxy-D-glucarate aldolase
- GenegarL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids256 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.
Catalytic activity
- 2-dehydro-3-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
- 5-dehydro-4-deoxy-D-glucarate = 2-hydroxy-3-oxopropanoate + pyruvate
- D-glyceraldehyde + pyruvate = 2-dehydro-3-deoxy-L-galactonate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 2/3.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 50 | Proton acceptor | |||
Site | 75 | Transition state stabilizer | |||
Site | 89 | Increases basicity of active site His | |||
Binding site | 151 | substrate | |||
Binding site | 153 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 179 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 179 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-dehydro-3-deoxyglucarate aldolase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | D-glucarate catabolic process | |
Biological Process | galactarate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-keto-4-deoxy-D-glucarate aldolase
- EC number
- Short namesKDGluc aldolase ; KDGlucA
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Dryocola
Accessions
- Primary accessionA0A9X3ADM0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homohexamer; trimer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length256
- Mass (Da)27,468
- Last updated2023-11-08 v1
- ChecksumBE995F8176193CC7
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JALHAP010000080 EMBL· GenBank· DDBJ | MCT4703113.1 EMBL· GenBank· DDBJ | Genomic DNA |