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A0A9X1K7C2 · A0A9X1K7C2_9ENTR

  • Protein
    5-keto-4-deoxy-D-glucarate aldolase
  • Gene
    garL
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Pathway

Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 2/3.

Features

Showing features for active site, site, binding site.

Type
IDPosition(s)Description
Active site50Proton acceptor
Site75Transition state stabilizer
Site89Increases basicity of active site His
Binding site151substrate
Binding site153Mg2+ (UniProtKB | ChEBI)
Binding site178substrate
Binding site179Mg2+ (UniProtKB | ChEBI)
Binding site179substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2-dehydro-3-deoxyglucarate aldolase activity
Molecular Functionmagnesium ion binding
Biological ProcessD-glucarate catabolic process
Biological Processgalactarate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5-keto-4-deoxy-D-glucarate aldolase
  • EC number
  • Short names
    KDGluc aldolase
    ; KDGlucA
  • Alternative names
    • 2-dehydro-3-deoxy-D-glucarate aldolase
    • 2-keto-3-deoxy-D-glucarate aldolase
    • 5-dehydro-4-deoxy-D-glucarate aldolase
    • Alpha-keto-beta-deoxy-D-glucarate aldolase

Gene names

    • Name
      garL
    • ORF names
      CTBON28M_17310

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • On28M
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Citrobacter

Accessions

  • Primary accession
    A0A9X1K7C2

Proteomes

Subcellular Location

Interaction

Subunit

Homohexamer; trimer of dimers.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-245HpcH/HpaI aldolase/citrate lyase

Sequence similarities

Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    256
  • Mass (Da)
    27,351
  • Last updated
    2023-11-08 v1
  • MD5 Checksum
    6ED80B6808614063E4476507E669234C
MNNTIFPNKFKAALAAQQIQIGCWSALASPISTEVLGLAGFDWLVLDGEHAPNDISTFIPQLMALKGSVSAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETEEEAVNAVASTRYPPEGIRGVSVSHRANMFGTVPDYFAQSNKNITIIVQIESQQGVDNVDAIAATEGVDGVFVGPSDLAAALGHLGNASHPDVQKAIQHIFARAKAHGKPSGILAPVEADARRYLEWGATFVAVGSDLGVFRSATQKLADSFKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAABKH010000006
EMBL· GenBank· DDBJ
MBW5274685.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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