A0A9X0PVY6 · A0A9X0PVY6_9ESCH

Function

function

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.
heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site29Involved in heme-bound ligand stabilization and O-O bond activation
Site84Influences the redox potential of the prosthetic heme and FAD groups
Binding site85Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); proximal binding residue
Active site95Charge relay system
Active site135Charge relay system
Binding site188FAD (UniProtKB | ChEBI)
Binding site204-207FAD (UniProtKB | ChEBI)
Binding site268-273NADP+ (UniProtKB | ChEBI)
Site388Influences the redox potential of the prosthetic heme and FAD groups
Binding site389-392FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionnitric oxide dioxygenase NAD(P)H activity
Molecular Functionoxygen binding
Molecular Functionoxygen carrier activity
Biological Processcellular response to nitrosative stress
Biological Processnitric oxide catabolic process
Biological Processresponse to toxic substance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Flavohemoprotein
  • Alternative names
    • Flavohemoglobin
    • Hemoglobin-like protein
    • Nitric oxide dioxygenase
      (NO oxygenase
      ; NOD
      ) (EC:1.14.12.17
      ) . EC:1.14.12.17 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      hmpA
    • Synonyms
      hmp
    • ORF names
      HEN21_007395

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 93.1518
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A9X0PVY6

Proteomes

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-134Globin
Region147-396Reductase
Domain150-255FAD-binding FR-type

Domain

Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.

Sequence similarities

Belongs to the globin family. Two-domain flavohemoproteins subfamily.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    396
  • Mass (Da)
    43,867
  • Last updated
    2023-11-08 v1
  • Checksum
    4D35EA55F5AFB016
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHLLATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAKTPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHNHANVGDVVKLVAPAGDFFMDVTNDTPVTLISAGVGQTPMLAMLDTLAKAGHSAQVNWFHAAENGDVHAFADEVKELGQALPRFTAHTWYRQPSETDRAKGQFDSEGLMDLSKLEGAFSDPAMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGPHKVL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JABWMT020000007
EMBL· GenBank· DDBJ
MBB2318113.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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