A0A9X0PUF2 · A0A9X0PUF2_9ESCH

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site456Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      HEN21_003425

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 93.1518
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A9X0PUF2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond344↔456Redox-active

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain404-480Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    54,987
  • Last updated
    2023-11-08 v1
  • Checksum
    0474292345950776
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILEKVVEEANNVDIREIAQQTEQEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNKTWLLWCERGVMSRLQALYLREQGFNNVKVYRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JABWMT020000003
EMBL· GenBank· DDBJ
MBB2317334.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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