A0A9W5W6S6 · A0A9W5W6S6_9BACL

  • Protein
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • Gene
    purL
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site57
Binding site60ATP (UniProtKB | ChEBI)
Binding site99ATP (UniProtKB | ChEBI)
Binding site101Mg2+ 1 (UniProtKB | ChEBI)
Binding site102-105substrate
Active site103Proton acceptor
Binding site124substrate
Binding site125Mg2+ 2 (UniProtKB | ChEBI)
Binding site248substrate
Binding site276Mg2+ 2 (UniProtKB | ChEBI)
Binding site320-322substrate
Binding site504ATP (UniProtKB | ChEBI)
Binding site541ATP (UniProtKB | ChEBI)
Binding site542Mg2+ 1 (UniProtKB | ChEBI)
Binding site544substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • ORF names
      BG53_05870

Organism names

  • Taxonomic identifier
  • Strain
    • CE1
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus

Accessions

  • Primary accession
    A0A9W5W6S6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain15-61Phosphoribosylformylglycinamidine synthase linker
Domain82-197PurM-like N-terminal
Domain210-364PurM-like C-terminal
Domain447-566PurM-like N-terminal
Domain580-719PurM-like C-terminal

Sequence similarities

Belongs to the FGAMS family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    747
  • Mass (Da)
    80,170
  • Last updated
    2023-11-08 v1
  • MD5 Checksum
    85EF07383A060E3C9B547B2F4E6D0EEA
MAQQLTAKEPTAEQIADQKIYTQFGVTDYEYELICGFLGRKPNYTEIGVFSVMWSEHCSYKNSKPVLRKFPVTGPRVLMGPGEGAGIVDIGDNQAVVFKIESHNHPSAVEPYQGAATGVGGIIRDIFSMGARPVALLNSLRFGKLENARVKYLFEHVVSGIAGYGNCIGIPTVAGEVMFDESYEGNPLVNAMCVGLIDHDKIQRGVAKGVGNPVFYAGPATGRDGIHGATFASVELSEESEEKRTAVQVGDPFMEKLVMEACLELINSGIVLGIQDMGAAGLTCSSAEMAAKAGNGLELYLDEVPQREEGMTPYEMMLSESQERMLFVVEPKHEAQAKEIFERWGIICAKVGKVTDDGRLRLFHKGEQVGDMPVKALVDECPIYNKPSREPAYYAENAAIDTSAYPEVTDLNDALRKVLASPTVASKEWVYNQYDYMVRTSTAVQPGSDAAVVAIRGTRKGLAMTTDCNGRYVYLDPEMGGRIAVAEAARNIVCSGAEPLAITDNLNFGNPEKPEVFWQIEKSVDGMAEACRVLDTPVIGGNVSLYNENAKGAIYPTPVVGMVGLVHDTDHITTQGFKAAGDIIILIGETKAELGGSELQYAVHGKTEGRPPALDLDLEKRVLDGVFGAIQKGLVASAHDLSEGGIAVALAESCISGGVGATVNITSGLRPDIALFSESQSRILLSVKPEKAADLKQWLANQGVTHTEIGTVGGRNLNVTVNGGNGIQSPVEQLEQVWKDAIPCLMK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JFHU01000185
EMBL· GenBank· DDBJ
EXX86573.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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