A0A9W5S290 · A0A9W5S290_9BACL

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site43(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site43dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site43isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site81(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site81dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site81isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site103[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site131(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site131dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site131isopentenyl diphosphate (UniProtKB | ChEBI)
Active site133Proton donor
Binding site170(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site198[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site228(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site228dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site228isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site271(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site271dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site271isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      BG53_15530

Organism names

  • Taxonomic identifier
  • Strain
    • CE1
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus

Accessions

  • Primary accession
    A0A9W5S290

Proteomes

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    317
  • Mass (Da)
    35,109
  • Last updated
    2023-11-08 v1
  • MD5 Checksum
    85DFFB7004B7E5930B93572191ECD7DD
MEIVKISPRGYCYGVVDAMVLALQTAKNLELPRPIYILGMIVHNQHVTDAFEKEGVITLDGANRLDILDQIDQGTVIFTAHGVSPEVRRIAREKGLAVVDATCPDVTKTHDLIREKVADGYEVVYIGKKGHPEPEGAIGIAPEHVHLIEREEEIDKLQVSSGKIIITNQTTMSQWDIKHLIAQLLERFPAAEIHNEICLATQVRQEAVAEQAGQAQLCIVVGDPRSNNSNRLAQVSEEIAGVKAYRVADVTEIRREWLAGISRVAVTSGASTPTPITKEVIAYLEQYDNDRPETWDVNRTVNMNKLLPTVREKSATL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JFHU01000094
EMBL· GenBank· DDBJ
EXX89473.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help