A0A9W5S1P2 · A0A9W5S1P2_9BACL
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids671 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 35-39 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 83-84 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 124 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 126 | N6-AMP-lysine intermediate | |||
Binding site | 147 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 181 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 293 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 317 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 410 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 413 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 426 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 432 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus
Accessions
- Primary accessionA0A9W5S1P2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length671
- Mass (Da)74,141
- Last updated2023-11-08 v1
- MD5 Checksum5315746E1CCF5A44CD5B930D312DB948
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JFHU01000109 EMBL· GenBank· DDBJ | EXX89058.1 EMBL· GenBank· DDBJ | Genomic DNA |