A0A9W5S004 · A0A9W5S004_9BACL

  • Protein
    Crossover junction endodeoxyribonuclease RuvC
  • Gene
    ruvC
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site7
Binding site7Mg2+ 1 (UniProtKB | ChEBI)
Active site67
Binding site67Mg2+ 2 (UniProtKB | ChEBI)
Active site140
Binding site140Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentHolliday junction resolvase complex
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease RuvC
  • EC number
  • Alternative names
    • Holliday junction nuclease RuvC
    • Holliday junction resolvase RuvC

Gene names

    • Name
      ruvC
    • ORF names
      BG53_08225

Organism names

  • Taxonomic identifier
  • Strain
    • CE1
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Paenibacillus

Accessions

  • Primary accession
    A0A9W5S004

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.

Family & Domains

Sequence similarities

Belongs to the RuvC family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    167
  • Mass (Da)
    18,044
  • Last updated
    2023-11-08 v1
  • Checksum
    26BDFDA660163C63
MRVLGIDPGIAIVGFGFIDKIGSRLVPVQYGSVETVAGTSHPTRLMQVYESIGALMDKYKPDAVAVEKLFFNRNVTTAFTVGEARGVIVLAAVQRGLPVAEYTPMQVKQAIVGYGKAEKRQVQEMTKMFLKLSAVPKPDDVADALAIGICHAHSAVLLDKMNGVTRR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JFHU01000221
EMBL· GenBank· DDBJ
EXX85535.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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