A0A9W5PLH2 · A0A9W5PLH2_BACCE

  • Protein
    tRNA-specific 2-thiouridylase MnmA
  • Gene
    mnmA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

137150100150200250300350
Type
IDPosition(s)Description
Binding site13-20ATP (UniProtKB | ChEBI)
Binding site39ATP (UniProtKB | ChEBI)
Active site104Nucleophile
Binding site128ATP (UniProtKB | ChEBI)
Site129Interaction with tRNA
Active site200Cysteine persulfide intermediate
Site341Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      IIU_05790

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • VD133
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group

Accessions

  • Primary accession
    A0A9W5PLH2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Structure

3D structure databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region99-101Interaction with target base in tRNA
Region150-152Interaction with tRNA
Domain208-270tRNA-specific 2-thiouridylase MnmA-like central
Domain282-357tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region308-309Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    371
  • Mass (Da)
    41,748
  • Last updated
    2023-11-08 v1
  • Checksum
    8D84A4C2B0629331
MNKLPHETRVVIGMSGGVDSSVAALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKQYWDKVFTYFLDEYRAGRTPNPDVMCNKEIKFKAFLEHAIALGADYVATGHYARVAYMDGEYKMLRGVDDNKDQTYFLNQLSQEQLSKTMFPLGELKKPQIREMAKEAGLATAAKKDSTGICFIGERNFKDFLSNYLPAQPGVMQTLSGEVKGKHDGLMYYTIGQRHGLGIGGNGDPWFAVGKNLKENILYVDQGFHNELLYGDEVIATNVGWVSNKEKETEFKCTAKFRYRQEDNKVTVQIVDENTVRILCDEPIRAITPGQAVVFYDGDECLGGATIDEVYRSGKKLDYLG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AHFB01000096
EMBL· GenBank· DDBJ
EOO28672.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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