A0A9Q4H940 · A0A9Q4H940_ACTPL

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site302Mg2+ (UniProtKB | ChEBI); catalytic
Binding site345Mg2+ (UniProtKB | ChEBI); catalytic
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      OYG11_07740

Organism names

Accessions

  • Primary accession
    A0A9Q4H940

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain39-119S1 motif
Region403-406Required for zinc-mediated homotetramerization and catalytic activity
Compositional bias572-589Basic and acidic residues
Region572-669Disordered
Compositional bias595-629Basic and acidic residues
Compositional bias650-662Basic and acidic residues
Region704-748Disordered
Compositional bias713-724Basic and acidic residues
Compositional bias725-737Basic residues
Compositional bias738-748Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    995
  • Mass (Da)
    112,973
  • Last updated
    2023-09-13 v1
  • MD5 Checksum
    39BF552B2B00D3CACCA4D8737F99532D
MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKGKITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPADYVFNGRPNIKDIIKEGQEVIVQVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDVPEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAIRDYLRRDIGEILIDNKKIFEKAKNHIRLVRPDFINRVRLYEGEVPLFSHYQIESQIESAFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFSRISRFGLLEMSRQRLSPSLSEASSHVCPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYLLNEKRKAISSIEKRHDVQVVVVPTESMETPHFSVYRLRENEVLPTLSYDLAKHYHDREEDTQLNTHTVSEEVLVTRNEPVITVESVLQSSELNLQPAPTPVEPAKPSLLSRLIAKVKSFFAPEEKAEEKPKPKARNQRDRRNRRERPNRVDRQERKQTQAQDEQKAQRQERNQERNEAKKARRQVVEEVAGVQETQTEQRSNKDNATTERRQRRDLRKKVRVENVEASVATTEPSTQPIEEKVQNIAQIQPLVAQETQPETVNEERQDVRENGRQRRLPRHLRVGNQRRRDNNNRKENVVAAMPLAAAVASPEMASGKIWVKFSEPVSTNKPNSKAEEKARFLSVDEMLEQQTTEVNEPIVESNIENNSASSVAPVVGFIKQQSDRDLVKENKARLHHQVEESEQADEQLVEKAMAKHVAPLGGFVSQHSDRDLVKEKNERVQKFQAQATQAPAIGNATAEAISVPVSNYQSDYSFNGRLGTFSRVMHTKAEMTVASAPEKALETVTVNEWTQSKYYFYGKGFGGHSSAVSHVHSAPRVANTDHE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias572-589Basic and acidic residues
Compositional bias595-629Basic and acidic residues
Compositional bias650-662Basic and acidic residues
Compositional bias713-724Basic and acidic residues
Compositional bias725-737Basic residues
Compositional bias738-748Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAPQFC010000001
EMBL· GenBank· DDBJ
MCY6524116.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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