A0A9Q0LGW9 · A0A9Q0LGW9_ANAIG
- ProteinPutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids321 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
Catalytic activity
- cytidine32/guanosine34 in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA + 2 S-adenosyl-L-homocysteine + 2 H+
RHEA-COMP:10246 CHEBI:74269 Position: 34CHEBI:82748 Position: 32+ 2 CHEBI:59789 = RHEA-COMP:10247 CHEBI:74445 Position: 34CHEBI:74495 Position: 32+ 2 CHEBI:57856 + 2 CHEBI:15378
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 53 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 55 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 81 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 97 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 122 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Active site | 162 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | tRNA (cytidine) methyltransferase activity | |
Biological Process | cytoplasmic translation | |
Biological Process | tRNA nucleoside ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metamonada > Anaeramoebidae > Anaeramoeba
Accessions
- Primary accessionA0A9Q0LGW9
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 21-204 | Ribosomal RNA methyltransferase FtsJ | |||
Compositional bias | 264-280 | Polar residues | |||
Region | 264-321 | Disordered | |||
Compositional bias | 281-296 | Basic and acidic residues | |||
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)36,312
- Last updated2023-09-13 v1
- MD5 ChecksumA9FAB7F35E73316A906399B87A092AFF
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 264-280 | Polar residues | |||
Compositional bias | 281-296 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAPDFW010000081 EMBL· GenBank· DDBJ | KAJ5072622.1 EMBL· GenBank· DDBJ | Genomic DNA |