A0A9P6R5P7 · A0A9P6R5P7_9FUNG

  • Protein
    Ubiquitin-related modifier 1
  • Gene
    RDH54_2
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionDNA translocase activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity
Biological Processdouble-strand break repair via homologous recombination
Biological Processprotein urmylation
Biological Processreciprocal meiotic recombination
Biological ProcesstRNA thio-modification
Biological ProcesstRNA wobble uridine modification

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-related modifier 1

Gene names

    • Name
      RDH54_2
    • Synonyms
      URM1
    • ORF names
      BGZ99_009897

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • REB-010B
  • Taxonomic lineage
    Eukaryota > Fungi > Fungi incertae sedis > Mucoromycota > Mortierellomycotina > Mortierellomycetes > Mortierellales > Mortierellaceae > Dissophora

Accessions

  • Primary accession
    A0A9P6R5P7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue, cross-link.

TypeIDPosition(s)Description
Modified residue4531-thioglycine
Cross-link453Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modification

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of UBA4, then thiocarboxylated (-COSH) via the rhodanese domain of UBA4.

Keywords

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain57-215Helicase C-terminal
Region318-341Disordered

Sequence similarities

Belongs to the URM1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    453
  • Mass (Da)
    50,316
  • Last updated
    2023-09-13 v1
  • Checksum
    B4EE5F2980AC1FA4
MQLAPTDPGSSRGMHFHFVVQLKDDPEIQGLYSGVKGLVEGSGNDMEDPSIGGKLCFVDALLRSIKKDTSERVVLVSNFTQTLDIFEKLCLRQKYGFFRLDGSTPTQKRQEMVDKFNAPSCQKFVFLLSAKSGGVGLNLVGASRLVMFDIDWNPSTDLQAMARIHRDGQSRPVYVYRLLSSGTIEEKMYQRQLTKIGLSDALMDGKTADKLNKFTAAELRDLFTFHDDEPCLTHSLLGCECIQGSASLAARGNSAKELSWDGIMDRTSASLAKRELKEWMHIDVDDWRLAPSGQSEQTQHGMDELGSKDRVLWNTILSGEGHDEEQEEEAGGEARGKRGKRGTRGPVTFVFYSGGMELLFDNQKTINTRVPAFFPSMAEAGEERHAATIRDLIAWTKQTLLRERPELFAGDDGSVRSGILVLVNGADWELEGELDYELQDGDEIVFISTLHGG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAAAIP010000879
EMBL· GenBank· DDBJ
KAG0311783.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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