A0A9P3JSA1 · A0A9P3JSA1_9VIRI

  • Protein
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
  • Gene
    PFP-BETA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by fructose 2,6-bisphosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site99diphosphate (UniProtKB | ChEBI)
Binding site197Mg2+ (UniProtKB | ChEBI); catalytic
Site198Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site224Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site225-227substrate
Active site227Proton acceptor
Binding site264-265substrate; ligand shared between dimeric partners
Binding site272-274substrate
Binding site333substrate
Binding site440-443substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processphotosynthesis
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
  • EC number
  • Short names
    PFP
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-PFK
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      PFP-BETA
    • ORF names
      CLOP_g18791

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • NIES-67
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Zygnemophyceae > Zygnematophycidae > Desmidiales > Closteriaceae > Closterium > Closterium peracerosum-strigosum-littorale complex

Accessions

  • Primary accession
    A0A9P3JSA1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Tetramer of two alpha (regulatory) and two beta (catalytic) chains.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain92-463Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    567
  • Mass (Da)
    60,766
  • Last updated
    2023-09-13 v1
  • Checksum
    5E414BFBCE2A1ED6
MVLSVALPAGLDASKLSKDISSYSELQLARLKYRPTLPSMLDGPSVVEKGPPTKAAGNDEEIAKLFPSLYGQPSVKLIPAGASAAAPTPITVGVVLSGGQAPGGHNVIAGLYDYLQEKVPGSKLLGFLGGPAGVMKCKYVEITAEFLYPYRNQGGFDIICSGRDKIESPEQFAMAFAMAVASAEKLSLDGLVVIGGDDSNTNACLLAEHFRGKGLKTKVIGCPKTIDGDLKAAQVPISFGFDTACKIYSETIGNVMTDARSGGKYYHFVRLMGRAASHITLECALQTHPNITLVGEEVFAKGMTLGQVTNHIADVICKRADAGKNFGVVLIPEGLIDFIPEINDLISQLNEILAKAAVDAEGMYEWKEGLQPNTRTLFDSLPKDIQDQLLLERDPHGNVQVARIETEKMLISMVEEELGRRAKAGQYKGAFEGRSHFCGYEGRCGMPTLFDATYCYALGSVAAALVQAGETGLIASVGNLTAPPQEWTVGGTALTELMVVERRKGKNKPVIKKAMVEFEGAPFKKFVSVRDDWALNDRYRSPGPVQFEGPTASQPNLTLLLEQGVSI

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A9P3JS62A0A9P3JS62_9VIRIPFP-BETA563

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BQMB01000004
EMBL· GenBank· DDBJ
GJP57557.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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