A0A9P2G965 · A0A9P2G965_CLOBO
- ProteinPyruvate carboxylase
- Genepyc
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1145 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 120 | ATP (UniProtKB | ChEBI) | |||
Binding site | 204 | ATP (UniProtKB | ChEBI) | |||
Binding site | 239 | ATP (UniProtKB | ChEBI) | |||
Active site | 296 | ||||
Binding site | 538 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 610 | substrate | |||
Binding site | 707 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 736 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 738 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 871 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionA0A9P2G965
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 707 | N6-carboxylysine | |||
Modified residue | 1111 | N6-biotinyllysine | |||
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length1,145
- Mass (Da)127,711
- Last updated2023-09-13 v1
- ChecksumBE159589D735DB54
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ACSJ01000001 EMBL· GenBank· DDBJ | EES92300.1 EMBL· GenBank· DDBJ | Genomic DNA |