A0A9L0RTE5 · A0A9L0RTE5_HORSE
- ProteinUMP-CMP kinase
- GeneCMPK1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids242 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Catalytic activity
- CMP + ATP = CDP + ADP
Cofactor
Note: Binds 1 Mg2+ ion per monomer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 45-50 | ATP (UniProtKB | ChEBI) | |||
Binding site | 71 | a ribonucleoside 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 93-95 | a ribonucleoside 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 125-128 | a ribonucleoside 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 132 | CMP (UniProtKB | ChEBI) | |||
Binding site | 166 | ATP (UniProtKB | ChEBI) | |||
Binding site | 172 | a ribonucleoside 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 183 | a ribonucleoside 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 211 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Cellular Component | nucleus | |
Molecular Function | (d)CMP kinase activity | |
Molecular Function | ATP binding | |
Molecular Function | UMP/dUMP kinase activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | phosphorylation | |
Biological Process | pyrimidine nucleotide biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUMP-CMP kinase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Perissodactyla > Equidae > Equus
Accessions
- Primary accessionA0A9L0RTE5
Proteomes
Organism-specific databases
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 65-95 | NMPbind | |||
Region | 165-175 | LID | |||
Domain
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Sequence similarities
Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length242
- Mass (Da)27,520
- Last updated2023-09-13 v1
- MD5 Checksum5241E1C83C0340421498F4F8D22300F8
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F7D3E3 | F7D3E3_HORSE | CMPK1 | 228 | ||
A0A9L0R7F8 | A0A9L0R7F8_HORSE | CMPK1 | 225 | ||
A0A5F5PYT1 | A0A5F5PYT1_HORSE | CMPK1 | 179 |
Keywords
- Technical term