A0A9J6PUN1 · A0A9J6PUN1_9ENTR

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site303Mg2+ (UniProtKB | ChEBI); catalytic
Binding site346Mg2+ (UniProtKB | ChEBI); catalytic
Binding site404Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site407Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      M8014_06750

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • H19S6
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Silvania

Accessions

  • Primary accession
    A0A9J6PUN1

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-119S1 motif
Region404-407Required for zinc-mediated homotetramerization and catalytic activity
Region511-827Disordered
Compositional bias589-740Basic and acidic residues
Compositional bias791-806Basic and acidic residues
Region1023-1068Disordered
Compositional bias1051-1068Polar residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,068
  • Mass (Da)
    119,874
  • Last updated
    2023-06-28 v1
  • Checksum
    E0DF8A52E0D51FE3
MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPSNYNAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALGSLELPDGMGLIVRTAGVGKSAEALQWDLGFRLKHWEAIQKAAESRPAPFLIHQESNVIVRAFRDYLRQDIGEILIDNPKVLELARQHIAALGRPDFTSKIKLYTGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTKEVHAIVPVPIASYLLNEKRAAVSAIETRLGGLRCVIVPNDQMQTPHYSVLRVRKGEETTTLSYMLPKLHEEEMALPSEAEYNERKLPEQPALAAFVMPEAPPAPAQESVDAQPVAQKPAAETRTAPAEPGLLSRFFGALKKMFAGEEPQPAPEPVEQKEVKPERSQDRRKRQNNRRDRNDRNDRRDNRDSRSDNSEGREGRENRDNRDNRESREGRESREGRDDNRRNRREKPQQNVEEREVRQPVSDESEKSRQRDEQQPRRERNRRRSDDKRQAQQETKELNREEQPVQDAEQEENIQVMPRRKPRQLNQKVRFQTQEEMAAEAVAPHAAPAQSTELAKVDLPAVIESAPEQEDNAENRDNTGMPRRSRRSPRHLRVSGQRRRRYRDERYPLQSPMPLAIACASPEMASGKAWIRYPVARPQEQQFDEQNIAPETVAPVMPVADEIITEAAAVSIPEPQVAAVETAHPEVIAAPVSEQPQIIAAEDVVVAEQVVEEVQAAEPVEIAAVAVEPEPQQDVVEVDVETNVQPEVEIADEPVPVAEAKPEVVEVKTAEVEVEVKPVPVAQANADVHYATAPMTRAPAPDYVPDAPRQSDWVRPAFDFEGKGSAGGHSASHQATAPATRPQSAE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias589-740Basic and acidic residues
Compositional bias791-806Basic and acidic residues
Compositional bias1051-1068Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAMGZK010000043
EMBL· GenBank· DDBJ
MCU6664039.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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