A0A9J6PR94 · A0A9J6PR94_9ENTR

Function

function

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.

GO annotations

AspectTerm
Molecular FunctionL-serine ammonia-lyase activity
Molecular Functionpyridoxal phosphate binding
Molecular Functionthreonine deaminase activity
Biological Processisoleucine biosynthetic process
Biological ProcessL-serine catabolic process
Biological Processthreonine catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    L-threonine dehydratase
  • EC number
  • Alternative names
    • Threonine deaminase

Gene names

    • Name
      ilvA
    • ORF names
      M8014_00860

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • H19S6
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Silvania

Accessions

  • Primary accession
    A0A9J6PR94

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain339-411ACT-like
Domain434-504ACT-like

Sequence similarities

Belongs to the serine/threonine dehydratase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    514
  • Mass (Da)
    56,198
  • Last updated
    2023-06-28 v1
  • Checksum
    296A1CFD9CA315BF
MAESQPLSAAPEGAEYLRAVLRAPVYEAVQVTPLQTMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKARGVITASAGNHAQGVAFSAARLGLKALIVMPVATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRVCREYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHVLSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKDACIFVGVRLSRGLEERKEILSLLHEGGYSVVDLSDDEMAKLHVRYMVGGRPSKPLQERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFELGEHEPDFETRLNELGYDCHDETHNPAFRFFLAG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAMGZK010000032
EMBL· GenBank· DDBJ
MCU6662903.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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