A0A9J6PR94 · A0A9J6PR94_9ENTR
- ProteinL-threonine dehydratase
- GeneilvA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids514 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.
Catalytic activity
- L-threonine = 2-oxobutanoate + NH4+
Cofactor
Pathway
Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | L-serine ammonia-lyase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | threonine deaminase activity | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | L-serine catabolic process | |
Biological Process | threonine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-threonine dehydratase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Silvania
Accessions
- Primary accessionA0A9J6PR94
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 339-411 | ACT-like | ||||
Sequence: ALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKDACIFVGVRLSRGLEERKEILSLLHEGGYSVVDLSD | ||||||
Domain | 434-504 | ACT-like | ||||
Sequence: RLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFELGEHEPDFETRLNELGYDCHDETH |
Sequence similarities
Belongs to the serine/threonine dehydratase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length514
- Mass (Da)56,198
- Last updated2023-06-28 v1
- Checksum296A1CFD9CA315BF