A0A9E9KU74 · A0A9E9KU74_9VIRU

Function

function

RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Cellular Componentvirion component
Molecular Functionhydrolase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Processcap snatching
Biological Processviral RNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase L
  • EC number
  • Short names
    Protein L

Organism names

  • Taxonomic identifier
  • Strain
    • Hainan40
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Arenaviridae > Mammarenavirus

Accessions

  • Primary accession
    A0A9E9KU74

Subcellular Location

Keywords

Interaction

Subunit

Homomultimer; the oligomeric structure is essential for the polymerase activity.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1177-1373RdRp catalytic

Sequence similarities

Belongs to the Bunyavirales RNA polymerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,228
  • Mass (Da)
    255,102
  • Last updated
    2023-05-03 v1
  • Checksum
    D7B5EBA4C58BA19C
MEEILTEARRLVGCHFPQMEQIARQKALFLAQREPKFTLIEGLKLLSLCIEIDSCRSNACDHNSESLSVEVLLFRNNILCPSLPYVVPDGFKLKGNVLILLECFVRSNPANFQQKYTEDLTKLESLRIDLGRAGVQLIPIVDGKTSFYTSIVPDWVCERFRHLLFKLLEYEQESNSEFEESEYQRLCESLSQNTSRLSGVDSLNILADHRSRHYEDIIQKCHVGINSSLSAREIRERVTMIFQTFRNRLRSQTVKHHFVKVNSEQLLKSFSELYESELSNAVDTMDSIRSEFNIVSPIIKFLYMRIPKESEKEEEIQTNGPNTLICGLRSMLNKVKSMKVLNTRRKMLLLFDTFILMAHIRYFKERSLYAEAEWLGSSFCSVNDRLVSLDETVQSLTKWVRARQRNHSKKLPERVTPTLNQVFQDMMRSTYKKIESALDYICMKPESYGVSFGALEIDIEELVAFKKDGVQPTMSYEALQAKETPYHVEMMSVQSDDDFRVLSSLCLSIVNSMKTSSVPKLRQNEMGASRYKVVRCREAFYQDLITRVGCFKLLYQKTGESSKCYAINDEKSGEICSFYADPKRYFLPIFSHEVLPETVRTMMSWLDGCEELKEHLADIHWLTKAIVILILCQPSKRSQRFLQNLRYFVMAMVSEYHHVKLMEKLREPLITKCEYYLYRLIRKLLTVLFDPSVKSLLTNRFKYMLNVSYFCHLITKETPDRLTDQIKCFEKFLEPKIDFGSCFINPSDVITDDELDTLSYGLDQFLAKPDISDSAGITHGKPGINKEIFSLMVSSFNAGLLFKESEMRGDFVDPLISSGCATALDLASNKSVVVNKFTEGGRVLEYDINKLTSAAVCELTETFSKKGKYLLNKDDYEYKVQRVISKLVTKGTTGTSVKEQEDVGIDDVFEGESKEFFYCVKQRVDNILANYQPGMKLQDTQPHQATLSDLSEFVSDPVKQRLIMSELSTHMVEDFDPNLLDESFYERFCKSVHDSAFKERYFYDTSLGPCPISLISRSVASRFFEAGEYFQCFKSVLLQMGGNRFSGKFMHHKHNNVNFKFDHSKLLDDVRISERESNSEALSKALSLSNCTSAALKNLCFYSEESPESFTSVGPNTGRLKFSLSYKEQVGGNRELYIGDLRTKMYTRLIEDYFEAFTKHFRGSCLNDEKEFENALIAMRLSVSLAQLSYSLDHSKWGPMMCPFLFLMLIQNIDLKSPSALEGIKSRDLISTLLCWHIHKMVEVPYNVISAMMRSYIKRNLGVMNSDHMTSTEAFIFNEFEIGVVPSHISSVLDMGQGILHNTSDFYGLVTEKFINYCLRLVSDGSVGSYTSSDDQISLFDSELTSLHDKSEEDFLCLLEFHNYLSDMLNKFISPKSVVGRFVAEFKSRFFVWGEEVPLLTKFVSASLHNVKCKEPHQLAETIDTIIDQSVANGVPVKLCNMMQDRTLALLRYAKYPLDPFLLFNKSDVKDWVDGTRGYRIMRNIENICPDQTGKIRSMLRMLYNKLKVGELHEEFTAVYLSSEPKESINKLMALVGKELLTDDDLSLCWLNLSTHHPLRMVLRQKVIYPSVINVEEEKVPTIIKTMQNKLSSHFTRGAQKLLSEAINKSAFQSSIASGFVGLCRTLGSKCVRDSNRGVHHIKSILEQLTTVSNVSHDQINGWDIWRVSNHSSNADNSGHDWVLTLLRPILWDYLCIALSTALEIGPWVLGDPKPKFEVKIRNKRSCDYFLLRPQNTRILEDKVSMNHLIHSVRRMYPEMFEKHLLPYMSDLAATKMKWSPRIKFLDLCVVLDVNCEALSLISHVVKWKRSEHYVVLMSELQESHDRQHVTLLDERVVSTENVSDNFMKQILFESFIRPIVVTSRTLGSFTWFPHKSAIPQGEGLGRLGPLSSFVEKVIFKGIERPMYEYDLSSGFSWVDLDVKPSIITAAELTRLKITEAGVFDDFWDLWSFIIQNSNEGFRVMKTIHVSVRSKGGSGSKNFFIHLQFNGSVDPLRMEVTMTLSEASYSGNVDLIFLESIWTLILTDPNFSSSCVSWYFSTETISDAIRNGAQVLGDLVLVDVGLERESLKLSGIEFERVGPDWEPVPLVLRDGYLWEGERKLVPLIVELHTDDLKVFIQELHEDHGQLLLESLACLSKTQLAQRVSFIHMDVIDALETVCGEAKGMLVLTEVMSQVGDWVDFKGNSICYSKSKGELMRQTPGGSLRLKGRLCEPFTQATVDVKEID

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
OP562167
EMBL· GenBank· DDBJ
WAU17171.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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