A0A9E7VAW3 · A0A9E7VAW3_9LILI
- ProteinDNA-directed RNA polymerase subunit alpha
- GenerpoA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids341 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit alpha
- EC number
- Short namesPEP
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Hydrocharitaceae > Vallisneria
Accessions
- Primary accessionA0A9E7VAW3
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-234 | Alpha N-terminal domain (alpha-NTD) | ||||
Sequence: MIREEVAGSTRTPQWKCVESRVDNKRLHYGRFILSPLRKDQASTIGICMRRALLGEIEETRIIHAKFDNVPHEYSMISGIEESVHEILMNLKEIVLRTHSHMDEVQDAFICTKGPKRVTAQDIISPPYVEIVNTTKHIANLRGPVDLCIELKIKRDRGYCIKSQNQSEGYPIDAVFMPVRNVNYSIHSYREESENEKQEILIFEIWTNGSLTPKEALYEASRNLIDLFIPFLHA | ||||||
Domain | 29-234 | DNA-directed RNA polymerase RpoA/D/Rpb3-type | ||||
Sequence: YGRFILSPLRKDQASTIGICMRRALLGEIEETRIIHAKFDNVPHEYSMISGIEESVHEILMNLKEIVLRTHSHMDEVQDAFICTKGPKRVTAQDIISPPYVEIVNTTKHIANLRGPVDLCIELKIKRDRGYCIKSQNQSEGYPIDAVFMPVRNVNYSIHSYREESENEKQEILIFEIWTNGSLTPKEALYEASRNLIDLFIPFLHA | ||||||
Region | 266-341 | Alpha C-terminal domain (alpha-CTD) | ||||
Sequence: QKEIAWKSIFIDQFQLSSRTYNCLKRSNIHTVLDLLSHSKEDLMRMEDFRAEDGKQILSILKERFAIDLPKNPFSF |
Domain
The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.
Sequence similarities
Belongs to the RNA polymerase alpha chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length341
- Mass (Da)39,828
- Last updated2023-05-03 v1
- Checksum78C5D3A75F77A89E