A0A9E7SIV7 · A0A9E7SIV7_9CAUD

Function

function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Regulated by specific antiholins that somehow sense superinfections and then delay lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Molecular Functionpore-forming activity
Biological Processviral release from host cell by cytolysis

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Holin

Gene names

    • ORF names
      PM133_00119

Organism names

Accessions

  • Primary accession
    A0A9E7SIV7

Proteomes

Subcellular Location

Host cell inner membrane
; Single-pass type II membrane protein
Note: Classified as a class III holin.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-34Cytoplasmic
Transmembrane31-49Helical
Topological domain50-218Periplasmic

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond175↔207

Post-translational modification

Disulfide bond is required for functionality.

Keywords

Interaction

Subunit

Homomultimer. Heterotetramer composed of 2 holin and 2 antiholin. The holin-antiholin complex binds dsDNA. Interacts (via C-terminus) with antiholin (via C-terminus); this interaction blocks the holin homomultimerization and delays host cell lysis. Interacts (via N-terminus) with the lysis inhibition accessory protein rIII; this interaction stabilizes the holin-antiholin complex thereby resulting in a robust block of the hole formation.

Family & Domains

Domain

The C-terminus serves, in association with the antiholin, as a DNA sensor for lysis inhibition under superinfection conditions.

Sequence similarities

Belongs to the T4likevirus holin family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    218
  • Mass (Da)
    25,208
  • Last updated
    2023-05-03 v1
  • Checksum
    2F5A2AD64240ECF6
MAAPRISFSPSDILFGVLDRLFKDNATGKVLASRVAVVILLFMMAIVWYRGDSFFEYYKQSKYETYSEIIEKERNARFESVALEQLQIVHISSEADFSAVYSFRPKNLNYFVDIIAYEGKLPSTISEKSLGGYPVDKTMDEYTVHLNGRHYYSDSKFAFLPTKKPTPEINYMYSCPYFNLDNIYAGTITMYWYRNDHISNDRLESICAQAARILGRAK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MZ234052
EMBL· GenBank· DDBJ
USM81463.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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