A0A9E4J719 · A0A9E4J719_UNCMY

Function

function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.
Uptake of L-lactate across the membrane. Can also transport D-lactate and glycolate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site677acetyl-CoA (UniProtKB | ChEBI)
Binding site684-685acetyl-CoA (UniProtKB | ChEBI)
Binding site839acetyl-CoA (UniProtKB | ChEBI)
Binding site876acetyl-CoA (UniProtKB | ChEBI)
Active site903Proton acceptor
Binding site903glyoxylate (UniProtKB | ChEBI)
Binding site995Mg2+ (UniProtKB | ChEBI)
Binding site995glyoxylate (UniProtKB | ChEBI)
Binding site1020-1023glyoxylate (UniProtKB | ChEBI)
Binding site1023Mg2+ (UniProtKB | ChEBI)
Binding site1104acetyl-CoA (UniProtKB | ChEBI)
Active site1193Proton donor

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular Functionlactate transmembrane transporter activity
Molecular Functionmagnesium ion binding
Molecular Functionmalate synthase activity
Biological Processglyoxylate catabolic process
Biological Processglyoxylate cycle
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Malate synthase G
  • EC number

Gene names

    • Name
      glcB
    • ORF names
      KA712_02455

Organism names

Accessions

  • Primary accession
    A0A9E4J719

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane47-74Helical
Transmembrane95-115Helical
Transmembrane121-140Helical
Transmembrane147-169Helical
Transmembrane189-211Helical
Transmembrane223-243Helical
Transmembrane314-337Helical
Transmembrane357-376Helical

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue1179Cysteine sulfenic acid (-SOH)

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region440-506Disordered
Compositional bias452-479Basic and acidic residues
Domain580-633Malate synthase N-terminal
Domain719-798Malate synthase G alpha-beta insertion
Domain899-1134Malate synthase TIM barrel
Domain1153-1242Malate synthase C-terminal

Sequence similarities

Belongs to the lactate permease family.
Belongs to the malate synthase family. GlcB subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,288
  • Mass (Da)
    137,966
  • Last updated
    2023-05-03 v1
  • Checksum
    03965EA1EE79862D
MAALPLLLGGVLLIGFRLSAKWSMATTFAIAAGIALGPWQVGMAHVAAASVSGLFAAFDILYIVFGALLLLHTLERSGGVNAIRRGFHAVSEDRRVQVVIVAWLFGSFIEGAAGFGAPAAIASPLMVAMGFPPACAVMLGMMIQSTAVTFGAVGTPVLLGVSVGFGEAFMTRSTAAGVDVLREVAARAASVHAIVGVFMPSLMCMMMTRFFGARRSWREGLGMLPFTLLGGVAFTLPYVLAAWFLGPEFPSLLGGLTGLATMTFVARKGWLRSRTTWDFPPRTEWPPSWGGHLNAKAEASAAAATSSLPAWKAWIPYVLLASLLVLTRVDLGLGAALKRVALVWPGIFGTNIVAKTTPLYLPGTILVLVVLITSRLHAMPGRALKQAFVESSRVLRGAGVVLCVHRSDGADPDPLRRQRRGLRQHAHRAGELGLAKCGRRVAGGRGRGGGARGLHRGLEHRVQHDARAVPVRRGPKPRDQWSHGGGAPGRGRGRGQHDRDSQRGGRLGQRGAFGGRGHHASKDTASHALLPHDGGDLGHGGGLWVAPRRPAPATERMNMHHDYIEVAGLKVAPALASLIRDEVAPGTGVTPEAFWQALAVLVRDFAPQNRALLDKRNELQAKLDAWHATHRGEAFNLPTYERFLKEIGYLVEPGPAFQIETANVDLEIARTAGPQLVVPVDNARYALNAANARWGSLYDALYGTNVVADPDNLRATAGFNATRGAQVVAEAMSLLDGIAPLEGGSWAEVTELSVGEGASPKALRLRLVGGQDTALVNPNLFVGFRLEAGRPSHVLLRHNGLHVEIALDAIHPVGKMHPAGIKDVTLEAALTTIADCEDSVAAVDAEDKVRVYRNWTGLMRGTLKARFDKGGKVVERGLAPDRTYVGVDGAALTLPGRSVLLVRNVGIHMYTDAVLSSEGEPIPEGLLDAMVTTLAARADLGGLSPLRNSRTGSVYIVKPKLHGPEEVAFTVAVFAAVERALGLAPNTIKMGIMDEERRTTTNLWACLRHARERVIFINTGFLDRTGDEIHSVMEAGPVVRKADMKNATWIRAYEDHNVDVGLAAGLAGRGQIGKGMWAMPDEMRAMMDTKAAHPRAGATTAWVPSPTAATLHALHYHQVDVAAVQAQLASRERAPLLSLLTPPVLTERPNAETIQAELENNAQGILGYVVRWIDQGVGCSKVPDMHDVGLMEDRATLRISSQHIANWLHHGLVTRAQVVATFEKVAALVDRQNAKDPAYQPMVPVEGNIAFAAALDLVFEGRQEPNGYTEPCLHRRRRERKTALRTAS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias452-479Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAGTJU010000001
EMBL· GenBank· DDBJ
MCG5051798.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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