A0A9E2K5W4 · A0A9E2K5W4_9MYCO

  • Protein
    Lysine--tRNA ligase
  • Gene
    lysX
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site1008Mg2+ 1 (UniProtKB | ChEBI)
Binding site1015Mg2+ 2 (UniProtKB | ChEBI)
Binding site1015Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular Functionacyltransferase activity
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular FunctiontRNA binding
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysX
    • Synonyms
      lysS
    • ORF names
      FGM25_08850

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ZLKRG26
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium

Accessions

  • Primary accession
    A0A9E2K5W4

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane12-33Helical
Transmembrane53-74Helical
Transmembrane81-100Helical
Transmembrane112-131Helical
Transmembrane143-166Helical
Transmembrane203-225Helical

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain772-1089Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,096
  • Mass (Da)
    119,172
  • Last updated
    2023-05-03 v1
  • Checksum
    5EC20965EBF16090
MTITTTRSRTRLRWVPAAAGWVLGVIATAALLSSVSPLIRHLTSAPREFIDNYLFNFPDTSFAWAFALAVMAGALAARKRIAWWVLVVILVVAVGFDVVSLTQRADTRFERVGEYLGLGFHIASIVVLVLARGEFWARVRRGALLKAAAVLVAGNVVGILLGWGLVELFPGSLQPDYRLAYTVNRVSGFATAASDLFVGRPPAILNALFGLFGALALMAAAVVLFQSQRADNALTGEDELAIRGLLESYGKNDSLGYFGTRRDKSVIFAPNGRAAITYRVEVGVCLASGDPIGDPRAWPQAIAAWLALCEAYGWAPGVMGASATAAQAFDAAGLSALQLGDEAILYPDRFRLSGPEMKPVRQAVTRARRAGVTVRLRRHRELSAGEMAEVISRADAWRDTETERGFSMALGRLGDSADGDCLLVEALDVPDGAAGGETVVGMLSFVPWGGNGLSLDLMRRSPQSPNGTVELMVSELLQRADTIGVTRVSLNFAMFRSAFEQGSQLGAGPVARLWRALLVFFSRWWQLETLYRSNMKYQPEWVPRFACYEDARLIPRVGVASVIAEGFLVLPFSRRGKQHTGQHSAVPHTVVDATAAAAAAPSEVDSPGDRVPDQVRVRMAKLQSLRDDGVDPYPVGRPPSHTVAAALVADDQDTISVAGRVMRLRDYGGVSFAQLRDWSGDVQLLVDKSGLDSAAPDFAGLDLGDLIEATGQMGYSKNGTRSVLVHRWRLLGKCLRPLPDKWKGLTDPEARVRARYVDLAVSPAARELITARSAILHSIRETLYSKGFVEVETPILQQIHGGANARPFRTHINAYDLDLYLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYRTWIDGCRELIQNAATAANGSMVVMRPDESAGGHGALEPVDISGTWAVKTVHGAVSEALGEQIDPETDLATLRRLCDAAGVPYLTHWDAGAVVLELYEHLVEGRTENPTFYIDFPASVSPLTRPHRSTPGLAERWDLVAWGTELGTAYSELTDPVEQRRRLQQQSTLAAGGDPEAMELDEDFLQAMEYAMPPTGGLGVGVDRVVMLLTGRSIRETLPFPLAKPR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VEQY01000028
EMBL· GenBank· DDBJ
MBU3686493.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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