A0A9E0X0M7 · A0A9E0X0M7_UNCPS
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids407 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 170-174 | GMP (UniProtKB | ChEBI) | ||||
Sequence: NHFIE | ||||||
Binding site | 171 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 188 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 280 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 280-281 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HN | ||||||
Binding site | 312-315 | GMP (UniProtKB | ChEBI) | ||||
Sequence: PGSM | ||||||
Binding site | 319 | GMP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 336 | GMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 336-339 | GMP (UniProtKB | ChEBI) | ||||
Sequence: HGAG | ||||||
Binding site | 406 | GMP (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA ligase activity | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Biological Process | DNA repair | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota
Accessions
- Primary accessionA0A9E0X0M7
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length407
- Mass (Da)44,794
- Last updated2023-05-03 v1
- ChecksumD61848E68984DCEB