A0A9D4X825 · A0A9D4X825_PEA
- ProteinATP-dependent 6-phosphofructokinase
- GenePFK
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids463 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 98 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 188 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 215-217 | substrate | ||||
Sequence: TID | ||||||
Active site | 217 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 260-262 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 316 | substrate | ||||
Sequence: E | ||||||
Binding site | 374-377 | substrate | ||||
Sequence: YMIR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Fabeae > Pisum
Accessions
- Primary accessionA0A9D4X825
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 6-27 | Helical | ||||
Sequence: YLRMFLTCLITSLIFLHILTHY |
Keywords
- Cellular component
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 92-398 | Phosphofructokinase | ||||
Sequence: CIVTCGGLCPGLNTVIRELVCGLYHMYGVHKVLGIEGGYRGFYSRNTIPLTPKIVNDIHKRGGTILKTSYGGHDTSKIVDSIQDRGINQVYILGGFGTQNEAAMIFEEIRRRGLKVAVVGIPKTIDNDIPVIDKSIGFDTAVEEAQRAINSAHVEAESAENGIGVVKLMGRCSGFIAMYATLASRDVDCCLIPESPFYLEGPGGVLEFIEKRLREQGHMVIVIAEGAGQELILPSDKSNKNRPDAASDDLFHDVGLWLSLKIKDHFARSKKMAINLKYIDPTYMIRAIPSNASDNVFCTLLAQSAVH |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length463
- Mass (Da)51,435
- Last updated2023-05-03 v1
- Checksum26E8F5E08557D0C0
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A9D4X5K1 | A0A9D4X5K1_PEA | PFK | 460 |
Keywords
- Technical term