A0A9D1VSX4 · A0A9D1VSX4_9FIRM

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site46Proton acceptor; for glutaminase activity
Active site115For glutaminase activity
Binding site121L-glutamine (UniProtKB | ChEBI)
Active site170Nucleophile; for glutaminase activity
Binding site197L-glutamine (UniProtKB | ChEBI)
Binding site203L-glutamine (UniProtKB | ChEBI)
Binding site351-358ATP (UniProtKB | ChEBI)
Binding site437deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site461ATP (UniProtKB | ChEBI)
Binding site466deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site471-474deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site597deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • ORF names
      H9737_01845

Organism names

Accessions

  • Primary accession
    A0A9D1VSX4

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-268CN hydrolase

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    636
  • Mass (Da)
    68,961
  • Last updated
    2023-05-03 v1
  • Checksum
    B8BA087519E942B6
MKHGFVRVAAATPRIRVADTRANAESVLGCLARAKEAGAELLVFPELCLCGYTCGDLFGQDVLLRGAAEALRKVAAATAGAGMLVFVGVPLQVNGVLYNCAAALNEGKVLAFIPKRHLPNYAEFYEKRNFQPYTGENIRVPFGGEEVPFGSKIIFRCRGEADFTVAAELCEDLWVPAPPSVSHALAGANIIVNLSASDETAGKAEYRRLLVRAQSAKTVSGYVYADAGDGESTTDMVFSGHDLICENGEILAESALFSNGILLSEIDVRRLAYERRRINTFYDASDLQGYEELPFAAGSAWEALTRPVSRLPFVPAEGGALDGRAELILSMQAAGLKKRLEHTRAETAVLGISGGLDSALALLVCARAFDALGRDRKGILAVTMPCFGTTDRTFQNSLRLMEALGVTSRTVPVADAVRGHFKDIGHDERVHNAAYENAQARMRTLVLMDLANDANGLVVGTGDLSELALGWATYNGDHMSMYGVNASVPKTLVKYLIRYEAARLGGGAQAVLEDVLATEISPELLPPEGGKIAQKTEELVGPYELHDFYLYHAIRWGFPPRKVFFLAQAAFAGKYEDAVLKKWLISFYRRFFSQQFKRSCLPDGVKVGSVALSPRGDWRMPSDASAALWLEEAEAL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DXFD01000030
EMBL· GenBank· DDBJ
HIX46415.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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