A0A9D1LTL8 · A0A9D1LTL8_9FIRM
- ProteinEndonuclease III
- Genenth
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids213 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA N-glycosylase activity | |
Molecular Function | DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | endonuclease activity | |
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, AP site formation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndonuclease III
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Candidatus Avimonoglobus
Accessions
- Primary accessionA0A9D1LTL8
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-186 | HhH-GPD | ||||
Sequence: LSAQCTDARVNIVTGPLFERYKTAEDFANADILELQGYIRSAGFYKNKSKNIIACCRRLVDVYGGEVPDNMEDLLTLAGTGRKTANLVLGDIFNKPAVVVDTHCIRLTNRIGLVKEQDPVKIEFALKKIVPEDYQNRFCHQLVMHGRA |
Sequence similarities
Belongs to the Nth/MutY family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length213
- Mass (Da)23,773
- Last updated2023-05-03 v1
- ChecksumF5B9014DD1A9B0DD