A0A977K9B3 · A0A977K9B3_9CREN

Function

function

A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for binding site, active site, site.

115320406080100120140
TypeIDPosition(s)Description
Binding site9Mg2+ (UniProtKB | ChEBI)
Active site29
Binding site40Mg2+ (UniProtKB | ChEBI)
Binding site53Mg2+ (UniProtKB | ChEBI)
Site55Transition state stabilizer

GO annotations

AspectTerm
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease Hjc
  • EC number
  • Short names
    Hjc
  • Alternative names
    • Holliday junction resolvase Hjc

Gene names

    • Name
      hjc
    • ORF names
      IPA_03710

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 13166
  • Taxonomic lineage
    Archaea > Thermoproteota > Thermoprotei > Desulfurococcales > Desulfurococcaceae > Ignicoccus

Accessions

  • Primary accession
    A0A977K9B3

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the Holliday junction resolvase Hjc family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    153
  • Mass (Da)
    17,385
  • Last updated
    2023-02-22 v1
  • Checksum
    09FC739B0D8698DB
MRRSATSYERDLARKLYKRGWVVMRAPASGAKVKRYPYPDLVALKRGYSIAVEVKTTSSERPIYIPKRQIEILKEWNVKGGAEPWIAVKVLDGRGWRFYSLDLLTETEKSYKLILEGGITLDELDSKAEGKAYVPLTHFLPKQSDGYTGTTAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP006868
EMBL· GenBank· DDBJ
UXD21385.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp