A0A977K975 · A0A977K975_9CREN

  • Protein
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • Gene
    purT
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site23-24N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site83N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site115ATP (UniProtKB | ChEBI)
Binding site156ATP (UniProtKB | ChEBI)
Binding site196-199ATP (UniProtKB | ChEBI)
Binding site204ATP (UniProtKB | ChEBI)
Binding site274Mg2+ (UniProtKB | ChEBI)
Binding site287Mg2+ (UniProtKB | ChEBI)
Binding site294N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site364N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site371-372N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionligase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylglycinamide formyltransferase 2 activity
Molecular Functionphosphoribosylglycinamide formyltransferase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • EC number
  • Alternative names
    • 5'-phosphoribosylglycinamide transformylase 2
    • Formate-dependent GAR transformylase
    • GAR transformylase 2
      (GART 2
      )
    • Non-folate glycinamide ribonucleotide transformylase
    • Phosphoribosylglycinamide formyltransferase 2

Gene names

    • Name
      purT
    • ORF names
      IPA_02470

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 13166
  • Taxonomic lineage
    Archaea > Thermoproteota > Thermoprotei > Desulfurococcales > Desulfurococcaceae > Ignicoccus

Accessions

  • Primary accession
    A0A977K975

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain120-316ATP-grasp

Sequence similarities

Belongs to the PurK/PurT family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    403
  • Mass (Da)
    44,564
  • Last updated
    2023-02-22 v1
  • Checksum
    A32ADE956E734C7B
MRNSLGTAWTSTANKALLLGSGELGKEVVIELQRLGVETIAVDRYDRAPAMHVAHKRYTINMLDYNQVVDVVRREHPDAVIPEVEAINLEALEDLEKEGYRIIPNAKAVGITMNRIRLRRFAAEQVGVPTTKYAFATNEDEAAEACAKVGFPCLIKPEMSSSGHGHTLARSEEEARKGFVEAIKHARGRSETVIVEEFVEIETELTALTYRHETGNGIETVPLIPVEHKRPKGIYYYYESWHPTTTPEEVTKKAQEIAKKVVEALGGVGIFGVEIIVTKDGRVLFSEVSPRPHDTGLVTLASLEQSEFAIHARAVMGLPVPEPKLVTPAATHVILAEEELEAPCIEGIGEALSVPHVQIRWFGKPRSYKERRMGLVIATGESVEEALKRAREAAGKVRVVKCR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP006868
EMBL· GenBank· DDBJ
UXD21294.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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