A0A975FJ86 · A0A975FJ86_LOWBP
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1366 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 212 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 214 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 226 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 229 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 601 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 603 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 605 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 988 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 1062 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 1069 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 1072 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mollicutes > Acholeplasmatales > Acholeplasmataceae > Candidatus Phytoplasma > 16SrVIII (Loofah witches'-broom group)
Accessions
- Primary accessionA0A975FJ86
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 375-655 | RNA polymerase N-terminal | ||||
Sequence: EWMVIDVLPVLPADLRPIVPLDGGRFATTDINDLYRRILNRNNRLKKQIKQNAPRLIIKNEKRMLQESVDSLFDNVKVNKKNNFNIEKSKTLKSLSEILRGKQGRFRQNLLGKRVDYSGRSVIIVGPELKIHQCGLPRIMAVILFKPFILNKLQEAKGIDKKSATVLYEKMDEYVFNVLEEVVKEHPVLLNRAPTLHRLGIQAFEPKLIDGKAIKLHPLVTPAFNADFDGDQMAVYLPLSIEAQAEARLLMLISNNILDPKNGNPVLFPSQDMVLGNYYLT |
Sequence similarities
Belongs to the RNA polymerase beta' chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,366
- Mass (Da)156,569
- Last updated2023-02-22 v1
- Checksum281E491616D7AB13
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP054393 EMBL· GenBank· DDBJ | QTX02847.1 EMBL· GenBank· DDBJ | Genomic DNA |