A0A974CYZ2 · A0A974CYZ2_XENLA

Function

function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.

Catalytic activity

  • oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H+ = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • a medium-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = a medium-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
    EC:1.3.8.7 (UniProtKB | ENZYME | Rhea)
  • decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.
  • dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H+ = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]
    This reaction proceeds in the forward direction.

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Lipid metabolism; mitochondrial fatty acid beta-oxidation.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site159-168FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site192-194FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site307-309FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site317-318FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site375-379FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Active site402Proton acceptor
Binding site404-406FAD (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionmedium-chain fatty acyl-CoA dehydrogenase activity
Biological Processfatty acid beta-oxidation
Biological Processmedium-chain fatty acid catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
  • EC number

Gene names

    • Name
      acadm.S
    • ORF names
      XELAEV_18025076mg

Organism names

  • Taxonomic identifier
  • Strain
    • J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    A0A974CYZ2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain43-152Acyl-CoA dehydrogenase/oxidase N-terminal
Domain158-256Acyl-CoA oxidase/dehydrogenase middle
Domain268-415Acyl-CoA dehydrogenase/oxidase C-terminal

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    422
  • Mass (Da)
    46,228
  • Last updated
    2023-02-22 v1
  • Checksum
    F4A4AF7A2E4DD3FC
MSALKVHRVLQHITRYGWRSQSTAPHAAASSSPGSPGFNFELSEQQKEFQATARKFAREEVMPVAAHYDKTGEYPVPLIKRAWELGLMNGHIPEQYGGLALGIFDTCLITEEIAYGCTGVQTAIEANSLGQMPVIIAGNEAQQKKYLGRMTEEPLMCAYCVTEPGAGSDVAGLKTRAEKKGNEYIINGQKMWITNGGKANWYFLLARTNPDPKVSAGKAFTGFIVDANSPGIQIGRKEMNMGQRCSDTRGIVFEDVRVPAENVLIGEGAGFKIAMGAFDKTRPPVAAGAVGLAQRALDEATKYAVERKTFGKLIAEHQAVSFLLAEMAMKVELARLAYQRAAWEIDCGRRNTYYASIAKAYAGDIANQVASDAVQVFGGNGFNSDYPVEKLMRDAKIYQIYEGTAQIQRLIISREHIGKYKE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM004473
EMBL· GenBank· DDBJ
OCT82544.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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