A0A962HEU7 · A0A962HEU7_9GAMM

Function

function

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site27-28NAD+ (UniProtKB | ChEBI)
Binding site48-49NAD+ (UniProtKB | ChEBI)
Binding site231S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site254Proton acceptor
Active site276Proton donor
Binding site307-309S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site312S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site337-338S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site389S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site418S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionNAD binding
Molecular Functionprecorrin-2 dehydrogenase activity
Molecular Functionsirohydrochlorin ferrochelatase activity
Molecular Functionuroporphyrin-III C-methyltransferase activity
Biological Processcobalamin biosynthetic process
Biological Processmethylation
Biological Processsiroheme biosynthetic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Siroheme synthase

Including 3 domains:

  • Recommended name
    Uroporphyrinogen-III C-methyltransferase
  • EC number
  • Short names
    Urogen III methylase
  • Alternative names
    • SUMT
    • Uroporphyrinogen III methylase
      (UROM
      )
  • Recommended name
    Precorrin-2 dehydrogenase
  • EC number
  • Recommended name
    Sirohydrochlorin ferrochelatase
  • EC number

Gene names

    • Name
      cobA
    • Synonyms
      cysG
    • ORF names
      KDI30_08570

Organism names

Accessions

  • Primary accession
    A0A962HEU7

Proteomes

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-208Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase
Domain124-148Siroheme synthase central
Domain155-211Sirohaem synthase dimerisation
Region222-469Uroporphyrinogen-III C-methyltransferase
Domain224-433Tetrapyrrole methylase

Sequence similarities

Belongs to the precorrin methyltransferase family.
In the C-terminal section; belongs to the precorrin methyltransferase family.
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    469
  • Mass (Da)
    50,776
  • Last updated
    2023-02-22 v1
  • Checksum
    A751DE468A213237
MKKSEKKFLPLFHQSEGKKALVVGGGAVAFRRCRILLDAGFVLTVVALAFDPVLKAMLEKHNAAIKQKAYEPDDIDNQYLVVAATDDVAVNQAIANVCKKRNIPVNVADNAHACDFVFSSVVERGPLKIAVSAGGKSPVLARLLKHLINGLVPSGYGELAGLVGEYRQKVRRAIPDEKQRIAFWESVLQGAVAEAVFSGKKAEAQSLLEKAVENPEKTIGAGEVYLIGAGPGDPGLLTLRAFRLIQQADVVLYDRLVSDEVMSLIRPDAEKIYVGKRRANHAVPQDDINRLLVQHAKQGKRVARLKGGDPFVFGRGGEEIEELSSESIPFQVIPGITAANGCAAYAGIPLTHRDHAQSVRFVTGQLKDGTVDLDWKTLVQPGQTLVFYMGLKGLPIIARELVLHGADKKLPIALIEKGTTMDQQVHIATLETIVDVLENASVKSPSLLIVGSVVSLHGKLQWFDKGRGH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAGRJA010000309
EMBL· GenBank· DDBJ
MCB1616053.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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