A0A962C4V5 · A0A962C4V5_9GAMM
- ProteinDihydrofolate synthase/folylpolyglutamate synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids265 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.
Catalytic activity
- (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H+ + phosphate
(6R)-5,10-methylenetetrahydrofolyl-(γ-L-Glu)(n) RHEA-COMP:13257 + CHEBI:30616 + CHEBI:29985 = (6R)-5,10-methylenetetrahydrofolyl-(γ-L-Glu)(n+1) RHEA-COMP:13258 + CHEBI:456216 + CHEBI:15378 + CHEBI:43474
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.
Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | tetrahydrofolylpolyglutamate synthase activity |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydrofolate synthase/folylpolyglutamate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales
Accessions
- Primary accessionA0A962C4V5
Proteomes
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)28,051
- Last updated2023-02-22 v1
- ChecksumEAFB5BEB0E1980C4
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: P |