A0A961MSI4 · A0A961MSI4_9RHOB

  • Protein
    tRNA-dihydrouridine(20/20a) synthase
  • Gene
    dusA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site87-89FMN (UniProtKB | ChEBI)
Binding site140FMN (UniProtKB | ChEBI)
Site167Interacts with tRNA
Active site170Proton donor
Binding site209FMN (UniProtKB | ChEBI)
Binding site241FMN (UniProtKB | ChEBI)
Site253Interacts with tRNA; defines subfamily-specific binding signature
Site256Interacts with tRNA
Binding site281-283FMN (UniProtKB | ChEBI)
Binding site304-305FMN (UniProtKB | ChEBI)
Site371Interacts with tRNA; defines subfamily-specific binding signature
Site374Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA dihydrouridine synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      dusA
    • ORF names
      KDK01_02120

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • HKST-UBA89
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Paracoccaceae

Accessions

  • Primary accession
    A0A961MSI4

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain85-380DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    401
  • Mass (Da)
    43,434
  • Last updated
    2023-02-22 v1
  • Checksum
    E8C56823092AC91C
MPHRAPLIALGNPGRNFATAICNPALIPVKFTHPCAEDREPGRGFVDARAALRYIWGIKLLSQPRLPRRVSQFPASIVEPWRLSVAPMLDWTDSICRQVHRRLSRHALLYTEMVTSAALVRGGARHLLRFDPVEHPVALQLGGSDPVELAQAVRMGADEGYSEINLNVGCPSDRVQSGSFGAVLMKSPELVGDCLGAMRAATRAEITVKCRIGVDDQDPEQALPALLDAARGAGVKRVIVHARKAWLQGLSPKENREVPPLDYALVERMLSAYPDLKLCLNGGIATLDQAEDLLSRGFAGVMIGRAAYHQPVDILASADRRIFGQPTTDADPVAVAHDMRPVIAGHLARGGRLIGATRHILGLFAGQPGARAWRRALSEMPNGTMADFDALLDGLTQRRAA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAGRMK010000056
EMBL· GenBank· DDBJ
MCB1405071.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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