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A0A949JZ85 · A0A949JZ85_9FIRM

  • Protein
    DNA ligase
  • Gene
    ligA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site80-81NAD+ (UniProtKB | ChEBI)
Active site104N6-AMP-lysine intermediate
Binding site125NAD+ (UniProtKB | ChEBI)
Binding site159NAD+ (UniProtKB | ChEBI)
Binding site297NAD+ (UniProtKB | ChEBI)
Binding site386Zn2+ (UniProtKB | ChEBI)
Binding site389Zn2+ (UniProtKB | ChEBI)
Binding site411Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      KTH89_07880

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ASD5720
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Lachnospirales > Lachnospiraceae > Diplocloster

Accessions

  • Primary accession
    A0A949JZ85

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain571-651BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    651
  • Mass (Da)
    73,182
  • Last updated
    2023-02-22 v1
  • MD5 Checksum
    8CC63139C15F980C09EA8D5F3EF30133
MAEQMERMKELVELLNRAGRAYYQEDREIMSNFEYDKLYDELVQMEKDTGVVLASSPTVHVGYEAVSELPKEQHESPMLSLDKTKSVDELQEWLKDQKAVLSWKLDGLTIVLTYRDGTLVKAVTRGNGEVGEVITNNARVFKNVPLSISYQGELILRGEAVITYSDFNRINDEIDDVDAKYKNPRNLCSGSVRQLNNEITARRNVHFMAFALVKADGVDFENSRRKQFEWLVDQGFDVVEHVMVDSGNLKEVVASFAERISDNDFPSDGLVLLLDDIDYGLSLGNTSKFPRNAMAFKWADEIRETRLTEIEWSPSRTGLINPVAIFEPVELEGTSVSRASVHNISIMKALQLGVGDTIEVYKANMIIPQIAENLTRSGVKDIPEVCPVCGGPTQIRQVNDVESLYCINPKCDAKRLKAFTLFVSRDALNIDGLSEMTLEKMIGRGYIHEFADLFHLSDYREEIVKMEGFGEKSYENLMASIENARNTTLPRVIYSLGISNIGLANAKMICREYQFDVEKMRQATAEELSGINGIGPVIAKSFVDYFADEENSLRFDHLLKELQIEKESGGEEEQVLEGKVFVITGSVEHFANRAELKEVIEAKGGKVTGSVTSKTDYLINNDTASASSKNKKARELNVPVISEQEFLDMIQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAHQCW010000010
EMBL· GenBank· DDBJ
MBU9736452.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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