A0A941SC70 · A0A941SC70_UNCPS

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site33Interaction with DNA
Site143Interaction with DNA
Site144Interaction with DNA
Site147Interaction with DNA
Site159Interaction with DNA
Active site307O-(5'-phospho-DNA)-tyrosine intermediate
Site309Interaction with DNA
Site504Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      JSR17_11325

Organism names

Accessions

  • Primary accession
    A0A941SC70

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain3-118Toprim
Region167-172Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    771
  • Mass (Da)
    87,097
  • Last updated
    2023-02-22 v1
  • Checksum
    561FC380E979C75E
MTNRLVVVESPAKAKTIKKYLGKGYQVMASYGHVRDLLPKEGAVDPNHDFAMKYVVIEKNANHVKAIADMLKKHDELLLATDPDREGEAIAYHLSELMREKGLLKTKPVRRVVFHEITKSAIVEAINHSRDLSMDLVNAQQARRALDYLVGFNLSPLLWRKVRPGLSAGRVQSPALRMIVERELEIESFIAKEYWTIEAHLEKQNQAFSAKLVELHGEKLKQFSITDSDMATKAVDDLKRDGKGKLQVKHIEKKKRKRNPSAPFTTSTLQQEASRKLGFGAQRTMRIAQQLYEGIDIGEGAIGLITYMRTDSVNLAQVALEEIRDVIKARYGNDALPDTPHTYKTKAKNAQEAHEAIRPTSAAVFPDDIKQALTADQYKLYNLIWKRTIACQMIPATIDTVAVDFACGQNHIFRANGSIVSVPGFLQVYEEGLDDAKKDKEGEGKMLPPLKEGDVLAIHEIQPHQHFTEPPPRYSEATLVKTLEEYGIGRPSTYATIIGTLQQREYAVLENKRFHPTDVGRVVNRFLTTYFTKYVDYGFTAGLEDELDQVSLGEKEWKPLLKEFWSPFITLVKEIQTNVQRKDVTQEAIEEKCPQCGSALSIRLGRRGRFIGCTSYPECDYTRSLEDKTGAEALATEAVVDRNCPDCGGTLQIKKGKYGKFIGCSNYPECKYIEPLEKPEDTGVQCPSCQKGNILKRKSRQGKIFFSCERYPDCKYALWYTPLAKPCPLCEWPITAIKTTKRNGTERVCPQKDCKFSEPYDVETEPEPEST

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAFECS010000039
EMBL· GenBank· DDBJ
MBS0287880.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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