A0A930UFI2 · A0A930UFI2_9PROT

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site14diphosphate (UniProtKB | ChEBI)
Binding site114Mg2+ (UniProtKB | ChEBI); catalytic
Site115Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site141Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site142-144substrate
Active site144Proton acceptor
Binding site190-192substrate
Binding site243substrate
Binding site293-296substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function6-phosphofructokinase activity
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      pfp
    • ORF names
      ISN26_05220

Organism names

Accessions

  • Primary accession
    A0A930UFI2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain9-317Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    415
  • Mass (Da)
    44,532
  • Last updated
    2023-02-22 v1
  • Checksum
    7A1B2169DC738B2D
MPDAPNLLYAQSGGVTSVINATAWGVATAAAASPKVGRVYAGRNGILGVLAEELIDVTAEDKREIAKLRHTPGGAFGSCRLKLTDPAKDSRSFERIAEVFKAHRIGYFLYNGGNDSQDTTNKVAEFCRARGVDVRCVGIPKTVDNDLAATDCCPGFGSVAKYVAATVAETALDVESMARTSTKLFILEVMGRHAGWIAAAGGLPYPAAGPVILALPEVPHKRFFAAVKAKIAKHGYCVVVASEGLADAKGRHLSERGTKDSFAHAQLGGVAAVLAERAKAKLGVKYHWALADYMQRAARHLSSQVDLDQAAKVGQAAVAYATAGKTQVMPAIVRTSDRPYRWTVKPVPLQEVANKERKLPKGYVTADGYYITAACRRYLQPLIAGEAPQPYRDGLPVHARLRRRAVRKKLPAFRV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JADHEI010000033
EMBL· GenBank· DDBJ
MBF2735462.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help