A0A927JE65 · A0A927JE65_9ACTN

Function

function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site154substrate
Binding site183substrate
Binding site212substrate
Binding site248substrate
Binding site268-270substrate
Binding site309-312substrate
Binding site348substrate
Binding site352Zn2+ (UniProtKB | ChEBI)
Binding site375substrate
Binding site416Zn2+ (UniProtKB | ChEBI)
Binding site496[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site499[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site504[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-carbon lyase activity
Molecular Functionzinc ion binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphomethylpyrimidine synthase
  • EC number
  • Alternative names
    • Hydroxymethylpyrimidine phosphate synthase
      (HMP-P synthase
      ; HMP-phosphate synthase
      ; HMPP synthase
      )
    • Thiamine biosynthesis protein ThiC

Gene names

    • Name
      thiC
    • ORF names
      HT102_12955

Organism names

  • Taxonomic identifier
  • Strain
    • G463
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Hoyosellaceae > Lolliginicoccus

Accessions

  • Primary accession
    A0A927JE65

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain19-81ThiC-associated

Sequence similarities

Belongs to the ThiC family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    556
  • Mass (Da)
    61,241
  • Last updated
    2023-02-22 v1
  • Checksum
    6CCCF13B57F7B914
MTLSSVHNPAEQAITTGPISGSSKIYVEVPGAPGLRVPMRRVHLTNGEHLDLYDTSGPYTDTSATIDIEQGLPRARDSWTKPAPIDGASTQLAWARAGIITDEMRFTAARESVSPELVRDEVARGRAVICANHKHPEIEPMIIGKAFSVKINGNIGNSAVTSSIGEEVEKMVWATRWGADTIMDLSTGKNIHETREWILRSSPVPVGTVPIYQALEKVKGDPTKLNWEIYRETIIEQCEQGVDYMTVHAGVLLRYIPLTAKRVTGIVSRGGSIMAAWCLAHHQESFLYTHFRELCEIFREYDVTFSLGDGLRPGSIADANDDAQFAELRTLGELTTIAKSHGVQVMIEGPGHVPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAKAGTAMLCYVTPKEHPGLPNRDDVKQGVIAYKIAAHSADLAKGHPRAQERDDALSRARFEFRWTDQFNLSLDPDPAREYHDETLPAEPAKTAHFCSMCGPKFCSMRISADVREYAERNGMNTQEDIDRVLAQGMAEKSAEFADHGNQVYLPLTTN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACYWE010000008
EMBL· GenBank· DDBJ
MBD8507391.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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