A0A927JCP7 · A0A927JCP7_9ACTN

Function

function

Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.

Miscellaneous

The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.
    EC:6.3.1.19 (UniProtKB | ENZYME | Rhea)

Pathway

Protein degradation; proteasomal Pup-dependent pathway.
Protein modification; protein pupylation.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site9Mg2+ (UniProtKB | ChEBI)
Binding site53ATP (UniProtKB | ChEBI)
Binding site55Mg2+ (UniProtKB | ChEBI)
Active site57Proton acceptor
Binding site63Mg2+ (UniProtKB | ChEBI)
Binding site66ATP (UniProtKB | ChEBI)
Binding site419ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionligase activity, forming carbon-nitrogen bonds
Molecular Functionmagnesium ion binding
Molecular Functionubiquitin-like protein transferase activity
Biological Processmodification-dependent protein catabolic process
Biological Processproteasomal protein catabolic process
Biological Processprotein pupylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pup--protein ligase
  • EC number
  • Alternative names
    • Proteasome accessory factor A
    • Pup-conjugating enzyme

Gene names

    • Name
      pafA
    • ORF names
      HT102_10430

Organism names

  • Taxonomic identifier
  • Strain
    • G463
  • Taxonomic lineage
    Bacteria > Bacillati > Actinomycetota > Actinomycetes > Mycobacteriales > Hoyosellaceae > Lolliginicoccus

Accessions

  • Primary accession
    A0A927JCP7

Proteomes

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    452
  • Mass (Da)
    51,043
  • Last updated
    2023-02-22 v1
  • MD5 Checksum
    66F1E6DE067CF3A25BC55F1AACAA1AFB
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDGLRQLVAHDRAGEMILEELLLDAEQRLSDEGIAGDIYLFKNNTDSAGNSYGCHENFLVSRSGEFSRISDVLLPFLVTRQLICGAGKVLHTVKAPIFTLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMAETTTLLKVGSAALVLEMIEAGVPFRDFALDNPIRAIREISHDITGTKPVRLAGGRQASALEIQREYHARAVEYLAAREPSDELDQVVDLWGRMLDAVESQDFSKVDTEIDWVIKRKLFTRYEERHGLPLGDPKIAQIDLAYHDIKQGRGVFDVLRRKGLVARVTSDEEVKQATALPPQTTRARLRGEFIAAAQDRGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVERLIAAM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACYWE010000005
EMBL· GenBank· DDBJ
MBD8506904.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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