A0A927JCP7 · A0A927JCP7_9ACTN
- ProteinPup--protein ligase
- GenepafA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids452 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Miscellaneous
The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.
Catalytic activity
Pathway
Protein degradation; proteasomal Pup-dependent pathway.
Protein modification; protein pupylation.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 9 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 53 | ATP (UniProtKB | ChEBI) | |||
Binding site | 55 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 57 | Proton acceptor | |||
Binding site | 63 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 66 | ATP (UniProtKB | ChEBI) | |||
Binding site | 419 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ligase activity, forming carbon-nitrogen bonds | |
Molecular Function | magnesium ion binding | |
Molecular Function | ubiquitin-like protein transferase activity | |
Biological Process | modification-dependent protein catabolic process | |
Biological Process | proteasomal protein catabolic process | |
Biological Process | protein pupylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePup--protein ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillati > Actinomycetota > Actinomycetes > Mycobacteriales > Hoyosellaceae > Lolliginicoccus
Accessions
- Primary accessionA0A927JCP7
Proteomes
Structure
Family & Domains
Sequence similarities
Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length452
- Mass (Da)51,043
- Last updated2023-02-22 v1
- MD5 Checksum66F1E6DE067CF3A25BC55F1AACAA1AFB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JACYWE010000005 EMBL· GenBank· DDBJ | MBD8506904.1 EMBL· GenBank· DDBJ | Genomic DNA |