A0A927JAU3 · A0A927JAU3_9ACTN
- ProteinEnergy-dependent translational throttle protein EttA
- GeneettA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids558 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ribosome binding | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA binding | |
Biological Process | negative regulation of translational elongation | |
Biological Process | translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnergy-dependent translational throttle protein EttA
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Hoyosellaceae > Lolliginicoccus
Accessions
- Primary accessionA0A927JAU3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associates with ribosomes and polysomes.
Keywords
- Cellular component
Interaction
Subunit
Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-256 | ABC transporter | ||||
Sequence: YTMKRVRKAHGDKVILDDVTLAFLPGAKIGVVGPNGAGKSSVLKIMAGLDQPSNGDAFIAPGKTVGILQQEPPLNEEKTVRQNVEEGLGELKVKLDRFNEIAELMATDYSDELMEEMGKLQEELDHADAWDIESQLEQAMDALRCPPPDEPVTQLSGGERRRVALCKLLLSKPDLLLLDEPTNHLDAESVLWLEQHLASYAGAVLAVTHDRYFLDHVAEWILELERGKAHGYEGNYSTYLEKKAERLEVQG | ||||||
Coiled coil | 281-308 | |||||
Sequence: QAKSKSRLARYEEMAAEAEKSRKLDMEE | ||||||
Domain | 322-539 | ABC transporter | ||||
Sequence: VEVKNLDKGYGDRMLIKDLSFTLPRNGIVGVIGPNGVGKTTLFKTIIGLEEADSGVVKIGDTVKLSYVDQHRGGIDPKKTVWQIVSEGLDFIHVGNTEMPSRAYVSAFGFKGPDQQKPAGVLSGGERNRLNLALTLKQGGNLILLDEPTNDLDVETLGSLENALEQFPGCAVVISHDRWFLDRTCTHILAWEGDETNEAKWFWFEGNFEAYEANKIER |
Domain
The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA.
The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1.
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length558
- Mass (Da)62,125
- Last updated2023-02-22 v1
- Checksum7A56E8B8A184BD53
Keywords
- Technical term